Mouse AKT2 Gene cDNA Clone / ORF Clone

AKT2 cDNA Clone Datasheet

AKT2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007434.3
• cDNA Size: 1446
• Gene Synonym: PKB; PKBbeta; AW554154; MGC14031; 2410016A19Rik; Akt2
• Source: Mouse

AKT2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus thymoma viral proto-oncogene 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1149 A/G not causing the amino acid variation.

AKT2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AKT2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

AKT2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG
61       ACCTGGAGGC CACGGTACTT CCTTCTGAAG AGTGATGGAT CTTTCATTGG GTATAAGGAG
121      AGGCCCGAGG CCCCTGACCA GACCTTACCC CCCCTGAACA ATTTCTCTGT AGCAGAATGC
181      CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG
241      ACCACAGTCA TCGAGAGGAC CTTCCATGTA GACTCTCCAG ATGAGAGGGA AGAGTGGATG
301      CGGGCTATCC AGATGGTCGC CAACAGTCTG AAGCAGCGGG GCCCAGGTGA GGACGCCATG
361      GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCTGTC
421      AACAAGGCAC GGGCCAAAGT GACCATGAAT GACTTCGATT ATCTCAAACT CCTCGGCAAG
481      GGCACCTTCG GCAAGGTCAT TCTGGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG
541      AAGATCCTGC GCAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG
601      AGCCGGGTTC TGCAGAATAC CAGGCACCCC TTCCTTACAG CCCTCAAGTA TGCCTTCCAG
661      ACCCATGACC GCCTATGCTT TGTGATGGAG TATGCCAACG GGGGTGAGCT GTTTTTCCAC
721      CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCGCGCT TTTATGGAGC AGAGATTGTG
781      TCAGCTCTGG AGTATTTGCA CTCGAGAGAT GTGGTGTACC GTGACATCAA GCTGGAAAAC
841      CTTATGTTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC
901      ATCAGTGATG GAGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG
961      GTGCTAGAGG ACAATGACTA TGGGCGAGCA GTGGACTGGT GGGGGCTGGG TGTGGTCATG
1021     TATGAGATGA TGTGTGGCCG CCTGCCATTC TACAACCAGG ACCACGAGCG CCTCTTTGAG
1081     CTCATTCTTA TGGAGGAGAT CCGCTTCCCG CGCACACTCG GGCCAGAGGC CAAGTCCCTG
1141     CTGGCTGGGC TGCTGAAGAA GGACCCAAAG CAGAGGCTCG GCGGAGGTCC CAGTGATGCG
1201     AAGGAGGTCA TGGAGCATAG ATTCTTCCTC AGCATCAACT GGCAGGACGT GGTACAGAAA
1261     AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT
1321     GACGAGTTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGAC
1381     CCGCTGGAAC TGGACCAGCG GACGCACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA
1441     GAGTGA

 

Quality Control:cDNA sequencing results is available upon request.