Anti-AK3L1 Antibody

Cat: 12406-MM08
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Anti-AK3L1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-AK3L1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human AK3L1
No cross-reactivity in ELISA with Insect cell lysate
Immunogen
Recombinant Human AK4 / AK3L1 protein (Catalog#12406-H20B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AK4 / AK3L1 (rh AK4 / AK3L1; Catalog#12406-H20B; P27144-1; Ala 2-Tyr 223). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #08
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-AK3L1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-AK3L1 Antibody (Mouse Monoclonal antibody) Images
Human AK4 / Adenylate Kinase 4 / AK3L1 Immunoprecipitation(IP) 6190

AK4 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

Lane C:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-AK4 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-AK4 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 26 kDa

Observed band size: 26 kDa

Human AK4 / Adenylate Kinase 4 / AK3L1 Western blot (WB) 14771

Anti-AK4 mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:26 kDa

Anti-AK3L1 Antibody Alternative Names
Anti-AK3 Antibody;Anti-AK3L1 Antibody;Anti-AK3L2 Antibody;Anti-AK4 Antibody
AK3L1 Background Information

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

Full Name
adenylate kinase 4
References
  • Xu G., et al.,(1992), Characterization of human adenylate kinase 3 (AK3) cDNA and mapping of the AK3 pseudogene to an intron of the NF1 gene. Genomics 13:537-542.
  • Gregory S.G., et al., (2006), The DNA sequence and biological annotation of human chromosome 1.Nature 441:315-321.
  • Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-AK3L1 Antibody Rabbit Monoclonal ELISA,IP 12406-R014
    Anti-AK3L1 Antibody Rabbit Polyclonal WB,ELISA,IP 12406-RP02
    Anti-AK3L1 Antibody Mouse Monoclonal ELISA 12406-MM04
    Anti-AK3L1 Antibody (PE) Mouse Monoclonal FCM 12406-MM09-P
    Anti-AK3L1 Antibody Rabbit Polyclonal ELISA 12406-RP01
    Anti-AK3L1 Antibody Mouse Monoclonal FCM,ICC/IF,IF 12406-MM09
    Anti-AK3L1 Antibody Rabbit Monoclonal WB,ICC/IF,IF,IP 12406-R010
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