AK4 / AK3L1 Antibody

AK4 / AK3L1 Antibody Datasheet

AK4 / AK3L1 Antibody Product Information

• Immunogen: Recombinant Human AK4 / AK3L1 protein (Catalog#12406-H20B)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 08
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AK4 / AK3L1 (rh AK4 / AK3L1; Catalog#12406-H20B; P27144-1; Ala 2-Tyr 223). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

AK4 / AK3L1 Antibody Usage Guide

• Specificity: Human AK4 / AK3L1
• No cross-reactivity in ELISA with

  Insect cell lysate

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human AK4 in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human AK4. The detection limit for Human AK4 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

AK4 / AK3L1 Antibody Background

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 (AK4 / AK3L1) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

References

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3. Liu,R. et al., 2009, Int J Biochem Cell Biol. 41 (6): 1371-80.
4. Miyoshi,K. et al., 2009, Acta Histochem Cytochem. 42 (2): 55-64.