AK2 Antibody (Antigen Affinity Purified)

AK2 Antibody ( Antigen Affinity Purified ) Datasheet

AK2 Antibody Product Information

• Immunogen: Recombinant Human AK2 protein (Catalog#13146-H20B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human AK2 (rh AK2; Catalog#13146-H20B; P54819-1; Met 1-Ile 239). AK2 specific IgG was purified by Human AK2 affinity chromatography.

AK2 Antibody Usage Guide

• Specificity: Human AK2
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human AK2 in human kidney with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human AK2 in human liver with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human AK2. The detection limit for Human AK2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

AK2 Antibody Background

Adenylate kinase 2 (AK2) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinase2 (AK2) is expressed in mitochondrial intermembrane space. It may play a role in apoptosis. It has been demonstrated that in apoptotic cells AK2 was translocated into the cytosol concomitantly with cytochronme C. Mutations in this gene are the cause of reticular dysgenesis. These mutations result in absent or strongly decreased protein expression. It has been demonstrated that restoration of AK2 expression in the bone marrow cells of individuals with reticular dysgenesis overcomes the neutrophil differentiation arrest, underlining its specific requirement in the development of a restricted set of hematopoietic lineages. It has been also established that AK2 is specifically expressed in the stria vascularis region of the inner ear, which provides an explanation of the sensorineural deafness in these individuals.

References

1. Lagresle-Peyrou C. et al., 2008, Nature Genetics. 41: 106-11.
2. Bruns GA. et al. 1977, 15 (5-6): 477-86.
3. K hler C. et al., 1999, FEBS Lett. 447 (1): 10-2.