AK1 Protein (His Tag)

Adenylate kinase 1 Protein Datasheet

AK1 Protein Product Information

• Synonym: RP11-203J24.1
• Protein Construction: A DNA sequence encoding the human AK1 (AAH01116.1) (Met 1-Lys 194) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

AK1 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human AK1 consisting of 209 amino acids and has a calculated molecular mass of 23.5 kDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, 10%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: AK1 protein

AK1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

AK1 Protein Description

Adenylate kinase 1 (AK1) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. AK1 K has functions in delivering AMP signals to metabolic sensors. It facilitates decoding of cellular information by catalyzing nucleotide exchange in the intimate "sensing zone" of metabolic sensors. With presence of AK1 in mitochondrial and myofibrillar compartments, the energy of two high-energy phosphoryls, γ- and β- phosphoryls in the ATP molecule is available. AK1 can read the cellular energy state, communicates AMP signals to metabolic sensors. This kinase is able to convey information about the overall energy balance. AK1 is related with several diseases, including nucleoside diphosphate kinase deficiency, hemolytic anemia, AK1 and post-ischemic coronary reflow and AK2 deficiency.

References

1. Lu Q. et al., 1996, Proc Natl Acad Sci. 93 (12): 5720-5.
2. Dzeja P. et al., 2009, Int J Mol Sci. 10 (4): 1729-72.
3. Lagresle-Peyrou C. et al., 2008, Nature Genetics. 41: 106-11.