Mouse AIMP1 / EMAP2 / SCYE1 Protein (Native) (Cytokine)

aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 Protein Datasheet

AIMP1 / EMAP2 / SCYE1 Protein Product Information

• Synonym: SCYE1, Aimp1, Emap2
• Protein Construction: A DNA sequence encoding the mouse SCYE1 (Q62386) (Ser145-Lys310) was expressed and purified.
• Source: Mouse
• Expression Host: E.coli

AIMP1 / EMAP2 / SCYE1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse SCYE1 consists of 166 amino acids and predicts a molecular mass of 18 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: AIMP1 / EMAP2 / SCYE1 protein

AIMP1 / EMAP2 / SCYE1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

AIMP1 / EMAP2 / SCYE1 Protein Description

aminoacyl tRNA synthase complex-interacting multifunctional protein 1, also known as Multisynthase complex auxiliary component p43, Endothelial monocyte-activating polypeptide II, AIMP1, EMAP2 and SCYE1, is a nucleus protein which contains one tRNA-binding domain. AIMP1 is a non-catalytic component of the multisynthase complex. AIMP1 negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. AIMP1 is involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. AIMP1 stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. It binds tRNA and possesses inflammatory cytokine activity. It promotes dermal fibroblast proliferation and wound repair and regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. AIMP1 plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations and induces maturation of dendritic cells and monocyte cell adhesion. AIMP1 modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.

References

1. Park S.G. et al., 1999,J Biol Chem. 274: 16673-6.
2. Shalak V. et al., 2001, J Biol Chem. 276: 23769-76.
3. Park S.G. et al., 2002,J Biol Chem. 277: 45243-8.
4. Park H. et al., 2002,J Leukoc Biol. 71: 223-30.
5. Tandle A.T. et al., 2009, Exp Cell Res. 315: 1850-9.