AIMP1 / EMAP II / SCYE1 Antibody (Antigen Affinity Purified) (Cytokine)

AIMP1 / EMAP II / SCYE1 Antibody ( Antigen Affinity Purified ) Datasheet

AIMP1 / EMAP II / SCYE1 Antibody Product Information

• Immunogen: Recombinant Human AIMP1 / EMAP II / SCYE1 protein (Catalog#10623-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human AIMP1 / EMAP II / SCYE1 (rh AIMP1 / EMAP II / SCYE1; Catalog#10623-H07E; NP_004748.2; Ala 2-Lys 312). AIMP1 / EMAP II / SCYE1 specific IgG was purified by Human AIMP1 / EMAP II / SCYE1 affinity chromatography.

AIMP1 / EMAP II / SCYE1 Antibody Usage Guide

• Specificity: Human AIMP1 / EMAP II / SCYE1
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human SCYE1 in human brain with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SCYE1.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

AIMP1 / EMAP II / SCYE1 Antibody Background

aminoacyl tRNA synthase complex-interacting multifunctional protein 1, also known as Multisynthase complex auxiliary component p43, Endothelial monocyte-activating polypeptide II, AIMP1, EMAP2 and SCYE1, is a nucleus protein which contains one tRNA-binding domain. AIMP1 is a non-catalytic component of the multisynthase complex. AIMP1 negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. AIMP1 is involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. AIMP1 stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. It binds tRNA and possesses inflammatory cytokine activity. It promotes dermal fibroblast proliferation and wound repair and regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. AIMP1 plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations and induces maturation of dendritic cells and monocyte cell adhesion. AIMP1 modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.

References

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3. Park S.G. et al., 2002,J. Biol. Chem. 277:45243-8.
4. Park H. et al., 2002,J. Leukoc. Biol. 71:223-30.
5. Tandle A.T. et al., 2009, Exp. Cell Res. 315:1850-9.