AGO2 Protein & Antibody (EIF2C2 Protein)

Argonaute 2 (Eukaryotic translation initiation factor 2C, 2)

AGO2 Products

AGO2 Protein, Recombinant

Molecule	Species	Description	Cat. No
AGO2/EIF2C2	Human	AGO2/EIF2C2 Protein, Recombinant	11079-H07B
AGO2/EIF2C2	Mouse	AGO2/EIF2C2 Protein, Recombinant	50683-M07B

  11079-H07B: Human AGO2 (11079-H07B) can bind Let-7a RNA and cleave target RNA (21nt). Activity Assay Protocol

AGO2 Antibody

Molecule	Application	Description	Cat. No
Mouse AGO2/EIF2C2	WB, ELISA	AGO2 Antibody (Antigen Affinity Purified)	50683-RP02

AGO2 cDNA Clone

Molecule	Species	Description	Cat. No
AGO2/EIF2C2	Human	Homo sapiens AGO2/EIF2C2 cDNA Clone(NM_012154.2)	HG11079-M
AGO2/EIF2C2	Mouse	AGO2/EIF2C2 cDNA Clone / ORF Clone	MG50683-M

AGO2 Related Areas

Signal Transduction>>Translational Regulator>>AGO2/EIF2C2

AGO2 Alternative Names

AGO2, EIF2C2, argonaute 2, argonaute2, eIF-2C 2, eIF2C 2, hAgo2, MGC3183, Q10, protein slicer [Homo sapiens]

Ago2, Eif2c2, argonaute 2, argonaute2, eIF-2C 2, eIF2C 2, mAgo2ENSMUSG00000072493, Gerp95, Gm10365, KIAA4215, mKIAA4215, protein slicer [Mus musculus]

AGO2 Related Products

Protein Products

Molecule	Species	Description
AGO1/EIF2C1	Human	AGO1/EIF2C1 Protein, Recombinant

cDNA Clone Products

Molecule	Species	Description
AGO1/EIF2C1	Human	Homo sapiens AGO1/EIF2C1 DNA ORF clone(NM_012199.2)

AGO2 Background

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

AGO2 Related Studies

1. O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.
2. Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.
3. Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.
4. Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.
5. Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.
6. Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.