Human AGA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13705-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

AGA cDNA Clone Datasheet

AGA cDNA Clone Product Information

Gene Bank Ref.ID:	BC012392
cDNA Size:	1041
Gene Synonym:	GA;AGU;ASRG;AGA
Source:	Human

AGA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens aspartylglucosaminidase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

AGA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AGA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

AGA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCGGA AGTCGAACTT GCCTGTGCTT CTCGTGCCGT TTCTGCTCTG CCAGGCCCTA
61       GTGCGCTGCT CCAGCCCTCT GCCCCTGGTC GTCAACACTT GGCCCTTTAA GAATGCAACC
121      GAAGCAGCGT GGAGGGCATT AGCATCTGGA GGCTCTGCCC TGGATGCAGT GGAGAGCGGC
181      TGTGCCATGT GTGAGAGAGA GCAGTGTGAC GGCTCTGTAG GCTTTGGAGG AAGTCCTGAT
241      GAACTTGGAG AAACCACACT AGATGCCATG ATCATGGATG GCACTACTAT GGATGTAGGA
301      GCAGTAGGAG ATCTCAGACG AATTAAAAAT GCTATTGGTG TGGCACGGAA AGTACTGGAA
361      CATACAACAC ACACACTTTT AGTAGGAGAG TCAGCCACCA CATTTGCTCA AAGTATGGGG
421      TTTATCAATG AAGACTTATC TACCAGTGCT TCTCAAGCTC TTCATTCAGA TTGGCTTGCT
481      CGGAATTGCC AGCCAAATTA TTGGAGGAAT GTTATACCAG ATCCCTCAAA ATACTGCGGA
541      CCCTACAAAC CACCTGGTAT CTTAAAGCAG GATATTCCTA TCCATAAAGA AACAGAAGAT
601      GATCGTGGTC ATGACACTAT TGGCATGGTT GTAATCCATA AGACAGGACA TATTGCTGCT
661      GGTACATCTA CAAATGGTAT AAAATTCAAA ATACATGGCC GTGTAGGAGA CTCACCAATA
721      CCTGGAGCTG GAGCCTATGC TGACGATACT GCAGGGGCAG CCGCAGCCAC TGGGAATGGT
781      GATATATTGA TGCGCTTCCT GCCAAGCTAC CAAGCTGTAG AATACATGAG AAGAGGAGAA
841      GATCCAACCA TAGCTTGCCA AAAAGTGATT TCAAGAATCC AGAAGCATTT TCCAGAATTC
901      TTTGGGGCTG TTATATGTGC CAATGTGACT GGAAGTTACG GTGCTGCTTG CAATAAACTT
961      TCAACATTTA CTCAGTTTAG TTTCATGGTT TATAATTCCG AAAAAAATCA GCCAACTGAG
1021     GAAAAAGTGG ACTGCATCTA A

Quality Control:cDNA sequencing results is available upon request.