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Human AFP Gene cDNA Clone (full-length ORF Clone)
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Human AFP Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG12177-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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AFP cDNA Clone Datasheet

 

AFP cDNA Clone Product Information

Gene Bank Ref.ID: BC027881

cDNA Size:

1830

Gene Synonym:

FETA; HPAFP;AFP

Source: Human

AFP cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens alpha-fetoprotein DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

AFP cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AFP cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

AFP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGTGGG TGGAATCAAT TTTTTTAATT TTCCTACTAA ATTTTACTGA ATCCAGAACA
61       CTGCATAGAA ATGAATATGG AATAGCTTCC ATATTGGATT CTTACCAATG TACTGCAGAG
121      ATAAGTTTAG CTGACCTGGC TACCATATTT TTTGCCCAGT TTGTTCAAGA AGCCACTTAC
181      AAGGAAGTAA GCAAAATGGT GAAAGATGCA TTGACTGCAA TTGAGAAACC CACTGGAGAT
241      GAACAGTCTT CAGGGTGTTT AGAAAACCAG CTACCTGCCT TTCTGGAAGA ACTTTGCCAT
301      GAGAAAGAAA TTTTGGAGAA GTACGGACAT TCAGACTGCT GCAGCCAAAG TGAAGAGGGA
361      AGACATAACT GTTTTCTTGC ACACAAAAAG CCCACTCCAG CATCGATCCC ACTTTTCCAA
421      GTTCCAGAAC CTGTCACAAG CTGTGAAGCA TATGAAGAAG ACAGGGAGAC ATTCATGAAC
481      AAATTCATTT ATGAGATAGC AAGAAGGCAT CCCTTCCTGT ATGCACCTAC AATTCTTCTT
541      TGGGCTGCTC GCTATGACAA AATAATTCCA TCTTGCTGCA AAGCTGAAAA TGCAGTTGAA
601      TGCTTCCAAA CAAAGGCAGC AACAGTTACA AAAGAATTAA GAGAAAGCAG CTTGTTAAAT
661      CAACATGCAT GTGCAGTAAT GAAAAATTTT GGGACCCGAA CTTTCCAAGC CATAACTGTT
721      ACTAAACTGA GTCAGAAGTT TACCAAAGTT AATTTTACTG AAATCCAGAA ACTAGTCCTG
781      GATGTGGCCC ATGTACATGA GCACTGTTGC AGAGGAGATG TGCTGGATTG TCTGCAGGAT
841      GGGGAAAAAA TCATGTCCTA CATATGTTCT CAACAAGACA CTCTGTCAAA CAAAATAACA
901      GAATGCTGCA AACTGACCAC GCTGGAACGT GGTCAATGTA TAATTCATGC AGAAAATGAT
961      GAAAAACCTG AAGGTCTATC TCCAAATCTA AACAGGTTTT TAGGAGATAG AGATTTTAAC
1021     CAATTTTCTT CAGGGGAAAA AAATATCTTC TTGGCAAGTT TTGTTCATGA ATATTCAAGA
1081     AGACATCCTC AGCTTGCTGT CTCAGTAATT CTAAGAGTTG CTAAAGGATA CCAGGAGTTA
1141     TTGGAGAAGT GTTTCCAGAC TGAAAACCCT CTTGAATGCC AAGATAAAGG AGAAGAAGAA
1201     TTACAGAAAT ACATCCAGGA GAGCCAAGCA TTGGCAAAGC GAAGCTGCGG CCTCTTCCAG
1261     AAACTAGGAG AATATTACTT ACAAAATGCG TTTCTCGTTG CTTACACAAA GAAAGCCCCC
1321     CAGCTGACCT CGTCGGAGCT GATGGCCATC ACCAGAAAAA TGGCAGCCAC AGCAGCCACT
1381     TGTTGCCAAC TCAGTGAGGA CAAACTATTG GCCTGTGGCG AGGGAGCGGC TGACATTATT
1441     ATCGGACACT TATGTATCAG ACATGAAATG ACTCCAGTAA ACCCTGGTGT TGGCCAGTGC
1501     TGCACTTCTT CATATGCCAA CAGGAGGCCA TGCTTCAGCA GCTTGGTGGT GGATGAAACA
1561     TATGTCCCTC CTGCATTCTC TGATGACAAG TTCATTTTCC ATAAGGATCT GTGCCAAGCT
1621     CAGGGTGTAG CGCTGCAAAC GATGAAGCAA GAGTTTCTCA TTAACCTTGT GAAGCAAAAG
1681     CCACAAATAA CAGAGGAACA ACTTGAGGCT GTCATTGCAG ATTTCTCAGG CCTGTTGGAG
1741     AAATGCTGCC AAGGCCAGGA ACAGGAAGTC TGCTTTGCTG AAGAGGGACA AAAACTGATT
1801     TCAAAAACTC GTGCTGCTTT GGGAGTTTAA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

alpha-fetoprotein (AFP) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"