- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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AFP / alpha-fetoprotein Antibody Datasheet
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AFP / alpha-fetoprotein Antibody Product Information
Recombinant Human AFP / alpha-fetoprotein protein (Catalog#12177-H08H)
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met 1-Val 609).
AFP / alpha-fetoprotein Antibody Usage Guide
Human AFP / alpha-fetoprotein
|Flow Cytometry :||
Flow cytometric analysis of Human AFP expression in HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-AFP (12177-MM24, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow cytometry was performed on a BD FACSCalibur flow cytometry system
Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
AFP / alpha-fetoprotein Antibody Related Products & Topics
AFP / alpha-fetoprotein Antibody Related Areas:
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|AFP||Human||AFP / alpha-fetoprotein Protein,Recombinant||12177-H08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human AFP||WB, ELISA||AFP / alpha-fetoprotein Antibody||12177-MM10|
|Human AFP||ELISA||AFP / alpha-fetoprotein Antibody||12177-MM15|
|Human AFP||FCM||AFP / alpha-fetoprotein Antibody||12177-MM24|
AFP / alpha-fetoprotein Antibody Background
- Mizejewski GJ. 2001, Exp Biol Med. 226 (5): 377-408.
- Tomasi TB. et al., 1977, Annual Review of Medicine. 28: 453-65.
- Leguy MC. et al., 2011, Ann Biol Clin. 69 (4): 441-6.