AFP / alpha-fetoprotein Antibody

AFP / alpha-fetoprotein Antibody Datasheet

AFP / alpha-fetoprotein Antibody Product Information

• Immunogen: Recombinant Human AFP / alpha-fetoprotein protein (Catalog#12177-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 24
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met 1-Val 609).

AFP / alpha-fetoprotein Antibody Usage Guide

• Specificity: Human AFP / alpha-fetoprotein
• Flow Cytometry:
  

  Flow cytometric analysis of Human AFP expression in HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-AFP (12177-MM24, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

AFP / alpha-fetoprotein Antibody Background

Alpha-fetoprotein (AFP) is a glycoprotein of 591 amino acids and a carbohydrate moiety belongning to the albuminoid gene superfamily. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. Alpha-fetoprotein (AFP) is first synthesized in the human by the yolk sac and liver (1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

References

1. Mizejewski GJ. 2001, Exp Biol Med. 226 (5): 377-408.
2. Tomasi TB. et al., 1977, Annual Review of Medicine. 28: 453-65.
3. Leguy MC. et al., 2011, Ann Biol Clin. 69 (4): 441-6.