Anti-Alpha-fetoprotein Antibody

Cat: 12177-R003
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Anti-Alpha-fetoprotein Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Alpha-fetoprotein Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Alpha-fetoprotein
Recombinant Human AFP / alpha-fetoprotein protein (Catalog#12177-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met1-Val609).
Monoclonal Rabbit IgG Clone #003
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Alpha-fetoprotein Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Alpha-fetoprotein Antibody (Rabbit Monoclonal antibody) Images
Human Alpha-fetoprotein/AFP Immunoprecipitation(IP) 14044

AFP was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-AFP rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-AFP rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 68 kDa

Observed band size: 68 kDa

Human Alpha-fetoprotein/AFP Western blot (WB) 14778

Anti-AFP rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:69 kDa

Observed band size:69 kDa

Anti-Alpha-fetoprotein Antibody Alternative Names
Anti-AFPD Antibody;Anti-Alpha-fetoprotein Antibody;Anti-FETA Antibody;Anti-HPAFP Antibody
Alpha-fetoprotein Background Information

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
  • Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
  • Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
  • Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Alpha-fetoprotein Antibody Mouse Monoclonal ICC/IF,IF 12177-MM27
    Anti-Alpha-fetoprotein Antibody Mouse Monoclonal ELISA(Cap) 12177-MM33
    Anti-Alpha-fetoprotein Antibody Rabbit Polyclonal ELISA 12177-RP01
    Anti-Alpha-fetoprotein Antibody Mouse Monoclonal FCM 12177-MM35T
    Anti-Alpha-fetoprotein Antibody Rabbit Polyclonal WB,ELISA,IP 12177-T52
    Anti-Alpha-fetoprotein Antibody Mouse Monoclonal WB,ELISA,IP 12177-MM10
    Anti-Alpha-fetoprotein Antibody (PE) Mouse Monoclonal FCM 12177-MM35-P
    Anti-Alpha-fetoprotein Antibody (APC) Mouse Monoclonal FCM 12177-MM35T-A
    Anti-Alpha-fetoprotein Antibody Mouse Monoclonal ELISA 12177-MM15
    Anti-Alpha-fetoprotein Antibody Mouse Monoclonal ELISA(Det) 12177-MM26
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