ADSL / Adenylosuccinate Lyase Protein (His Tag)

Adenylosuccinate lyase Protein Datasheet

ADSL / Adenylosuccinate Lyase Protein Product Information

• Synonym: RP5-1042K10.8, AMPS, ASASE, ASL
• Protein Construction: A DNA sequence encoding the human ADSL (P30566-1) (Met 1-Leu 484) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

ADSL / Adenylosuccinate Lyase Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human ADSL comprises 500 amino acids and has a predicted molecular mass of 57 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, 10%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ADSL / Adenylosuccinate Lyase protein

ADSL / Adenylosuccinate Lyase Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ADSL / Adenylosuccinate Lyase Protein Description

Adenylosuccinate lyase, also known as adenylosuccinase, ADSL and ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the β-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by defferent mutations to the enzyme. More than 30 different mutations have been found worldwide. The mutations resulting in gene defects are spread throughout the enzyme, with the majority located far from the active site.

References

1. Nassogne M. et al., 2000, Brain and development. 22 (6): 383-6.
2. Sivendran S. et al., 2004, J Biol Chem. 279 (51): 53789-97.
3. Lee TT. et al., 1999, Biochemistry. 38 (1): 22-32.