Anti-ADK Antibody

Cat: 13149-RP02
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Anti-ADK Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ADK Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human ADK
Immunogen
Recombinant Human ADK protein (Catalog#13149-H20B)
Preparation
Produced in rabbits immunized with purified, recombinant Human ADK (rh ADK; Catalog#13149-H20B; AAH03568.1; Met 1-His 345). ADK specific IgG was purified by Human ADK affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ADK Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ADK Antibody (Rabbit Polyclonal antibody) Images
Human ADK Western blot (WB) 13533

Anti-ADK rabbit polyclonal antibody at 1:500 dilution

Lane A: RAW264.7 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: NIH/3T3 Whole Cell Lysate

Lane D: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:40 kDa

Observed band size:40 kDa

(We are unsure as to the identity of these extra bands.)

Anti-ADK Antibody Alternative Names
Anti-AK Antibody
ADK Background Information

Adenosine kinase(ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (ADK), which differ at their N-terminal ends, are found in mammalian cells. It has been shown that the two ADK isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE). It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo. 

Full Name
adenosine kinase
References
  • Aronica E, et al. (2011) Upregulation of adenosine kinase in astrocytes in experimental and human temporal lobe epilepsy. Epilepsia.52 (9): 1645-55.
  • Kuettel S, et al. (2011) Crystal structures of T. b. rhodesiense adenosine kinase complexed with inhibitor and activator: implications for catalysis and hyperactivation. PLoS Negl Trop Dis. 5 (5): e1164.
  • Cui XA, et al. (2011) Molecular characterization of Chinese hamster cells mutants affected in adenosine kinase and showing novel genetic and biochemical characteristics. BMC Biochem. 12 (1): 22.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ADK Antibody Rabbit Monoclonal ELISA 13149-R001
    info info
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