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>Rabbit PAb Antibody
>ADK Antibody, Rabbit PAb, Antigen Affinity Purified
|Catalog||Size (Price)||Quantity||In Stock||Operation|
ADK Antibody (Antigen Affinity Purified) Datasheet
|Order or Inquire for ADK Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.00975 ng/well in ELISA|
ADK Antibody Product Information
Recombinant Human ADK protein (Catalog#13149-H20B)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
Produced in rabbits immunized with purified, recombinant Human ADK (rh ADK; Catalog#13149-H20B; AAH03568.1; Met 1-His 345). ADK specific IgG was purified by Human ADK affinity chromatography.
ADK Antibody Usage Guide
|Western blot :||
This antibody can be used at 2-5 μg/mL with the appropriate secondary reagents to detect Human ADK in WB.
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ADK. The detection limit for Human ADK is approximately 0.00975 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
ADK Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|ADK||Human||ADK Protein,Recombinant , with GST Tag||13149-H20B|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human ADK||WB, ELISA||ADK Antibody||13149-RP02|
ADK Antibody Background
Adenosine kinase (ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (AdK), which differ at their N-terminal ends, are found in mammalian cells. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE).It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo. Both siRNA and amiRNA mediated silencing of ADK led to impaired root growth, small, crinkled rosette leaves and reduced apical dominance.
- Aronica E. et al., 2011, Epilepsia. 52 (9): 1645-1655.
- Kuettel S. et al., 2011, PLoS Negl Trop Dis. 5 (5): 1164.
- Cui XA. et al., 2011, BMC Biochem. 12 (1): 22.