Mouse ADCK1 Gene cDNA Clone / ORF Clone

ADCK1 cDNA Clone Datasheet

ADCK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_028105.3
• cDNA Size: 1578
• Gene Synonym: 2610005A10Rik; Adck1
• Source: Mouse

ADCK1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus aarF domain containing kinase 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 395 A/G resulting in the amino acid Gln substitution by Arg.

ADCK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ADCK1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ADCK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAGAA AGGCTCTCAA GCTTGCTTCA TGGACCAGCG TGGCTCTTGC TGCCTCCGGT
61       GTCTACCTCT ACAGTAACAA CTACTTGGAC CCTAATGACT TTGGCGCTGT CAGGGTGGGC
121      AGAGCTGTTG CTACGACAGC TGTCATCAGC TATGACTACC TCACCTCCCT GAGGAGTGTC
181      CCATATGGCT CTGAGGAGTA TTTGCAGCGT CGATCCCAGG TGCACCTCCG CTCTGCCAGG
241      CGTCTCTTTG AGCTCTGCTG TGCCAACCGG GGCACTTTCA TCAAGGTGGG CCAGCACCTG
301      GGGGCGCTGG ACTACCTGCT GCCAGAAGAG TACACCAGCA CACTGAAGGT GTTGCACAGT
361      CAAGCCCCAC AGAGCAGCAT GCAAGAGGTC CGGCGGGTCA TCCGAGAAGA CCTGGGCAAG
421      GAGATCCACG ATTTGTTCCT GAGCTTCGAT GACACCCCTC TTGGGGCAGC CTCCCTGGCC
481      CAGGTCCACA AGGCGGTGTT GCATGATGGT CGGACAGTGG CAGTTAAGGT CCAGCACCCG
541      AAGGTTCAGG CTCAAAGCTC TAAGGACATT CTCCTGATGG AGGTGCTTGT CCTGGCCGTG
601      AAGCAACTTT TCCCAGATTT TGAATTCATG TGGCTGGTGG ATGAAGCGAA GAAGAACCTG
661      CCTCTCGAGT TGGACTTCCT GAATGAAGGG AGGAACGCTG AGAAAGTGGC CCACATGCTC
721      AGGCACTTTG ACTTCCTAAA GGTTCCCCAG ATCCACTGGG AGCTGTCTAC CAAGAGGGTG
781      CTCCTGATGG AATTTGTAGA GGGAGGTCAA GTCAACGACA GGGCCTACAT GGAGAAGAAC
841      CAGATCGATG TGAATGAGAT CTCCTGCCAC CTGGGCAAGA TGTACAGTGA GATGATCTTT
901      GTCAATGGCT TCGTGCACTG TGACCCCCAC CCAGGCAACG TACTGGTACG GAAGCGTCCT
961      GACACGGGCA AGGCTGAGAT TGTCCTCTTA GACCATGGGC TTTACCAGGT GCTCACGGAG
1021     GAGTTCCGCC TGGACTACTG CCATCTGTGG CAGTCTCTGA TCTGGACTGA CATGGACGGG
1081     CTGAAACAGT ACAGCCAGCG CCTGGGAGCT GCAGACCTCT ACCCACTGTT TGCCTGTATG
1141     CTGACAGCCC GGTCCTGGGA CTCAGTCAAA CAGGGCATTG GGCAAGCTCC AGTCTCTGCT
1201     ACTGAGGACT CAGAGATTCG CAATAATGCA GCCTGCTACC TGCCTGAGAT CAGCCAGCTC
1261     CTTAACCATG TGCCTCGCCA GATGCTGCTC ATCCTGAAGA CTAATGATCT ACTCCGTAGC
1321     ATTGAGACCA CCCTGGGCAC GCGCTCCAGT GCCAGTTCCT TCCTCAACAT GTCTCGGTGT
1381     TGTATCAGGG CGCTGGCTGA ACACAAGAAG AGGGATGCCG GCTCTTTCTT CAGAAGGACT
1441     CAGATATCTT TCAGTGAGGC CTTTAGCCTG TGGCAGATCA ACCTTCATGA ACTTCTGCTT
1501     CGAGTGAGGG CCTTGAGGCT AGCTTGCTGG GTCTCAGCTC TCCTGGGCTG GCTGACTCGG
1561     GCTCCACACA GAATGTGA

 

Quality Control:cDNA sequencing results is available upon request.