Rat ADAM17 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80350-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ADAM17 cDNA Clone Datasheet

ADAM17 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_020306.1
cDNA Size:	2484
Gene Synonym:	TACE; Adam17
Source:	Rat

ADAM17 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus ADAM metallopeptidase domain 17 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ADAM17 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ADAM17 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ADAM17 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGCAGC GTCTCCTCTT CCTGACCACT TTGGTGCCTT TCGTCCTGGC ACCCCGACCT
61       CCGGAGGAAC CAGGCTCTGG CTCCCACCTG CGACTTGAGA AGCTTGATTC TTTGCTCTCA
121      GACTACGATA TCCTCTCTTT ATCTAATATC CAGCAGCACT CCATAAGGAA AAGGGATCTA
181      CAGTCTGCGA CACACTTAGA AACACTACTA ACTTTTTCAG CTTTGAAAAG GCATTTTAAA
241      TTATACTTGA CATCAAGTAC AGAACGCTTT TCACAGAACT TGCGAGTCGT GGTGGTGGAT
301      GGGAAAGAGG AAAGCGAGTA CAGTGTGAAG TGGCAGGACT TCTTCAGCGG ACATGTGGTT
361      GGTGAGCCTG ACTCTAGGGT TCTAGCGCAC ATAGGAGATG ATGATGTCAC AGTAAGAATC
421      AACACAGATG GGGCAGAATA TAACATAGAG CCACTTTGGA GGTTTGTTAA TGATACTAAA
481      GATAAAAGGA TGCTGGTGTA TAAGTCTGAA GATATCAAGG ATTTTTCACG TTTGCAGTCT
541      CCAAAAGTAT GTGGTTATTT AAATGCAGAT AGTGAAGAGT TGCTTCCAAA AGGGCTCATA
601      GACAGAGAGC CATCTGAAGA GTTTGTCCGT CGAGTGAAGA GGCGAGCTGA ACCTAACCCA
661      TTGAAGAATA CTTGTAAATT ACTGGTGGTG GCAGATCATC GATTTTATAA GTACATGGGC
721      CGAGGAGAAG AGAGCACTAC TACAAATTAC TTAATAGAGT TAATTGACCG AGTTGATGAC
781      ATATACCGGA ACACCTCGTG GGACAATGCA GGATTTAAAG GTTATGGAGT ACAGATAGAA
841      CAGATTCGAA TTCTCAAGTC TCCACAAGAG GTAAAACCTG GTGAAAGACA CTTCAATATG
901      GCAAAAAGTT TTCCAAATGA AGAAAAGGAT GCTTGGGATG TGAAGATGCT GCTGGAGCAA
961      TTTAGCCTTG ATATAGCTGA AGAGGCCTCT AAAGTCTGCC TGGCTCATCT TTTCACCTAC
1021     CAAGATTTTG ATATGGGAAC TCTTGGATTA GCTTACGTTG GTTCTCCCAG AGCAAACAGT
1081     CATGGAGGGG TTTGTCCAAA AGCTTATTAC AACCCAGGTG TGAAGAAGAA CATCTATTTG
1141     AATAGTGGTC TGACAAGTAC AAAAAATTAT GGTAAAACCA TCCTTACAAA GGAGGCTGAC
1201     CTGGTTACAA CTCATGAATT GGGACACAAT TTTGGAGCAG AACATGATCC TGATGGGCTG
1261     GCAGAGTGTG CCCCAAATGA GGACCAAGGA GGAAAGTATG TTATGTACCC CATAGCTGTG
1321     AGTGGTGACC ATGAGAATAA TAAGATGTTT TCAAACTGCA GTAAACAGTC CATCTACAAG
1381     ACCATAGAAA GCAAGGCTCA AGAGTGCTTC CAGGAGCGCA GCAACAAGGT GTGCGGCAAC
1441     TCCAGGGTGG ACGAAGGAGA AGAGTGTGAC CCGGGCATCA TGTACCTGAA CAACGACACC
1501     TGCTGCAATA GTGACTGCAC ACTGAAGCCA GGTGTGCAGT GCAGTGATAG GAATAGTCCT
1561     TGCTGTAAAA ATTGTCAGTT TGAGACGGCC CAGAAGAAGT GCCAGGAGGC TATCAATGCT
1621     ACTTGCAAAG GAGTGTCTTA CTGCACAGGG AATAGCAGTG AGTGCCCCCC ACCAGGAGAT
1681     GCTGAAGATG ACACTGTGTG CTTGGACCTG GGCAAGTGCA AGGCTGGGAA ATGCATCCCT
1741     TTCTGCAAGA GGGAGCAGGA GCTGGAGTCC TGCGCATGTG CTGACACCGA CAACTCGTGC
1801     AAGGTATGCT GCAGGAACCT TTCTGGCCCA TGTGTGCCTT ACGTCGATGC AGAGCAAAAG
1861     AACTTATTTT TGAGGAAAGG GAAGCCCTGT ACAGTAGGGT TTTGTGACAT GAATGGCAAA
1921     TGTGAGAAAC GGGTACAGGA CGTAATTGAG CGGTTTTGGG ATTTCATTGA CCAGCTGAGC
1981     ATCAACACTT TTGGGAAGTT TCTGGCAGAC AACATCGTTG GGTCTGTTCT GGTTTTCTCC
2041     TTGATATTTT GGATTCCTTT CAGCATTCTT GTCCACTGTG TGGATAAGAA ACTGGACAAG
2101     CAGTATGAGT CCCTGTCTCT GTTTCATCAC AGTAACATTG AGATGTTGAG CAGCATGGAC
2161     TCAGCATCTG TTCGCATTAT CAAGCCGTTC CCTGCACCCC AGACTCCAGG TCGTCTGCAG
2221     GCCCTGCAGC CGGCTGCAAT GATGCCGCCA GTGTCTGCGG CTCCAAAACT GGATCACCAG
2281     AGGATGGACA CCATCCAGGA AGACCCCAGC ACAGATTCAC ATGTGGATGA TGATGGCTTT
2341     GAGAAGGACC CCTTCCCCAA CAGCAGTGCA GCCGCCAAGT CCTTTGAGGA CCTCACAGAC
2401     CACCCAGTCA CCAGGAGTGA GAAGGCCGCC TCCTTCAAGC TGCAGCGTCA GAGCCGAGTT
2461     GATAGCAAAG AGACAGAGTG TTAG

Quality Control:cDNA sequencing results is available upon request.