Human ADAM10 Gene cDNA Clone / ORF Clone

Human ADAM10 cDNA Clone Datasheet

ADAM10 cDNA Clone Product Information

• Gene Bank Ref.ID: BC126253.1
• cDNA Size: 1539
• Gene Synonym: kuz; AD10; MADM; CD156c; HsT18717; ADAM10
• Source: Human

ADAM10 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ADAM metallopeptidase domain 10 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide (1512-1537) insertion mutation

ADAM10 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ADAM10 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ADAM10 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTGTTGC TGAGAGTGTT AATTCTGCTC CTCTCCTGGG CGGCGGGGAT GGGAGGTCAG
61       TATGGGAATC CTTTAAATAA ATATATCAGA CATTATGAAG GATTATCTTA CAATGTGGAT
121      TCATTACACC AAAAACACCA GCGTGCCAAA AGAGCAGTCT CACATGAAGA CCAATTTTTA
181      CGTCTAGATT TCCATGCCCA TGGAAGACAT TTCAACCTAC GAATGAAGAG GGACACTTCC
241      CTTTTCAGTG ATGAATTTAA AGTAGAAACA TCAAATAAAG TACTTGATTA TGATACCTCT
301      CATATTTACA CTGGACATAT TTATGGTGAA GAAGGAAGTT TTAGCCATGG GTCTGTTATT
361      GATGGAAGAT TTGAAGGATT CATCCAGACT CGTGGTGGCA CATTTTATGT TGAGCCAGCA
421      GAGAGATATA TTAAAGACCG AACTCTGCCA TTTCACTCTG TCATTTATCA TGAAGATGAT
481      ATTAACTATC CCCATAAATA CGGTCCTCAG GGGGGCTGTG CAGATCATTC AGTATTTGAA
541      AGAATGAGGA AATACCAGAT GACTGGTGTA GAGGAAGTAA CACAGATACC TCAAGAAGAA
601      CATGCTGCTA ATGGTCCAGA ACTTCTGAGG AAAAAACGTA CAACTTCAGC TGAAAAAAAT
661      ACTTGTCAGC TTTATATTCA GACTGATCAT TTGTTCTTTA AATATTACGG AACACGAGAA
721      GCTGTGATTG CCCAGATATC CAGTCATGTT AAAGCGATTG ATACAATTTA CCAGACCACA
781      GACTTCTCCG GAATCCGTAA CATCAGTTTC ATGGTGAAAC GCATAAGAAT CAATACAACT
841      GCTGATGAGA AGGACCCTAC AAATCCTTTC CGTTTCCCAA ATATTGGTGT GGAGAAGTTT
901      CTGGAATTGA ATTCTGAGCA GAATCATGAT GACTACTGTT TGGCCTATGT CTTCACAGAC
961      CGAGATTTTG ATGATGGCGT ACTTGGTCTG GCTTGGGTTG GAGCACCTTC AGGAAGCTCT
1021     GGAGGAATAT GTGAAAAAAG TAAACTCTAT TCAGATGGTA AGAAGAAGTC CTTAAACACT
1081     GGAATTATTA CTGTTCAGAA CTATGGGTCT CATGTACCTC CCAAAGTCTC TCACATTACT
1141     TTTGCTCACG AAGTTGGACA TAACTTTGGA TCCCCACATG ATTCTGGAAC AGAGTGCACA
1201     CCAGGAGAAT CTAAGAATTT GGGTCAAAAA GAAAATGGCA ATTACATCAT GTATGCAAGA
1261     GCAACATCTG GGGACAAACT TAACAACAAT AAATTCTCAC TCTGTAGTAT TAGAAATATA
1321     AGCCAAGTTC TTGAGAAGAA GAGAAACAAC TGTTTTGTTG AATCTGGCCA ACCTATTTGT
1381     GGAAATGGAA TGGTAGAACA AGGTGAAGAA TGTGATTGTG GCTATAGTGA CCAGTGTAAA
1441     GATGAATGCT GCTTCGATGC AAATCAACCA GAGGGAAGAA AATGCAAACT GAAACCTGGG
1501     AAACAGTGCA GTCCAAGTCA AGGTCCTTGT TGTACAGCAC AGTGTGCATT CAAGTCAAAG
1561     TCTGA

 

Quality Control:cDNA sequencing results is available upon request.