Cynomolgus monkey ACVR2B Gene cDNA Clone / ORF Clone (Cytokine)

ACVR2B cDNA Clone Datasheet

ACVR2B cDNA Clone Product Information

• cDNA Size: 1650
• Gene Synonym: ACVR2B
• Source: Cynomolgus monkey

ACVR2B cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) activin A receptor, type IIB DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide (19-123) insertion mutation and the point mutation 912 G/A not causing the amino acid variation.
  Please check the sequence information before order.

ACVR2B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACVR2B cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ACVR2B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTGGCC TGCGTCCAGG GGTGAGTGCA GGGGGTTGGT GACCCCAACC CACCACCCGG
61       CCTCCCTCCC TCAGGGTGGC CTGGCACCCA GGACTGGGAG TAGGGGGGTT TGGGTGGTGG
121      TCCCAGGGGC ATGCTCAGTG GTTCTCTTTT CTCTGGGGCA CAGGCTCTGG GCGTGGGGAG
181      GCTGAGACAC GGGAGTGCAT CTACTACAAC GCCAACTGGG AGCTGGAGCG CACCAACCAG
241      AGCGGCCTGG AGCGCTGCGA GGGCGAGCAG GACAAGCGGC TGCACTGCTA CGCCTCCTGG
301      CGCAACAGCT CTGGCACCAT CGAGCTCGTG AAGAAGGGCT GCTGGCTAGA TGACTTCAAC
361      TGCTATGATA GGCAGGAGTG TGTGGCCACT GAGGAGAACC CCCAGGTGTA CTTCTGCTGC
421      TGTGAAGGCA ACTTCTGCAA CGAGCGCTTC ACTCATTTGC CAGAGGCTGG GGGCCCGGAA
481      GTCACGTATG AGCCACCTCC GACAGCCCCC ACCCTGCTCA CGGTGCTGGC CTACTCACTG
541      CTGCCCATTG GGGGCCTTTC CCTCATCGTC CTGCTGGCCT TTTGGATGTA CCGGCATCGC
601      AAGCCCCCGT ACGGTCATGT GGACATCCAT GAGGACCCTG GGCCTCCACC CCCATCCCCT
661      CTGGTGGGCC TGAAGCCACT GCAGCTGCTG GAGATCAAGG CTCGGGGGCG CTTTGGCTGT
721      GTCTGGAAGG CCCAGCTCAT GAATGACTTT GTAGCTGTCA AGATCTTCCC ACTCCAGGAC
781      AAGCAGTCGT GGCAGAGTGA ACGGGAGATC TTCAGCACAC CTGGCATGAA GCACGAGAAC
841      CTGCTACAGT TCATTGCTGC CGAGAAGCGA GGCTCCAACC TCGAAGTAGA GCTGTGGCTC
901      ATCACGGCCT TCCACGACAA GGGCTCCCTC ACGGATTACC TCAAGGGGAA CATCATCACA
961      TGGAACGAAC TGTGTCATGT AGCAGAGACG ATGTCACGAG GCCTCTCATA CCTGCATGAG
1021     GACGTGCCCT GGTGCCGTGG CGAGGGCCAC AAGCCGTCTA TTGCCCACAG GGACTTTAAA
1081     AGTAAGAATG TATTGCTGAA GAGCGACCTC ACAGCCGTGC TGGCTGACTT TGGCTTGGCT
1141     GTTCGATTTG AGCCAGGGAA ACCTCCAGGG GACACCCACG GACAGGTAGG CACGAGACGG
1201     TACATGGCTC CTGAGGTGCT CGAGGGAGCC ATCAACTTCC AGAGAGATGC CTTCCTGCGC
1261     ATTGACATGT ATGCCATGGG GCTGGTGCTG TGGGAGCTTG TGTCTCGCTG CAAGGCTGCA
1321     GACGGACCCG TGGATGAGTA CATGCTGCCC TTTGAGGAAG AGATTGGCCA GCACCCTTCG
1381     TTGGAGGAGC TGCAGGAGGT GGTGGTGCAC AAGAAGATGA GGCCCACCAT TAAGGATCAC
1441     TGGTTGAAAC ACCCGGGCCT GGCCCAGCTT TGTGTGACCA TCGAGGAGTG CTGGGACCAT
1501     GATGCAGAGG CTCGCTTGTC TGCGGGCTGT GTGGAGGAGC GGGTGTCCCT GATTCGGAGG
1561     TCGGTCAACG GCACTACCTC GGACTGTCTC GTTTCCCTGG TGACCTCTGT CACCAATGTG
1621     GACCTGCCCC CTAAAGAGTC AAGCATCTAA

 

Quality Control:cDNA sequencing results is available upon request.