ACVR2B / ACTRIIB Antibody

ACVR2B / ACTRIIB Antibody Datasheet

ACVR2B / ACTRIIB Antibody Product Information

• Immunogen: Recombinant Mouse ACVR2B protein (Catalog#50173-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 307
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ACVR2B extracellular domain (rM ACVR2B; Catalog#50173-M08H; NP_031423.1; Met 1-Thr 134).

ACVR2B / ACTRIIB Antibody Usage Guide

• Specificity: Mouse ACVR2B / ACTRIIB
• No cross-reactivity in ELISA with
  

  Human ACVR2B

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ACVR2B. The detection limit for Mouse ACVR2B is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ACVR2B / ACTRIIB Antibody Background

Activin A receptor, type I IB, also known as ACVR2B, is an activin type 2 receptor. Activins are dimeric growth and differentiation factors belonging to the TGF-beta superfamily. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type I I (II and IIB) receptors. Mouse ACVR2B is a transmembrane proteins consisting of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane segment, a cytoplasmic domain with predicted serine/threonine specificity, and as well as a C-terminal PDZ binding motif. Mouse ACVR2B shares 99% aa sequence identity with human and rat within ECD. Type I receptors are essential for signaling, and type I I receptors are required for binding ligands and for expression of type I receptors. On ligand binding, Type I and II receptors form a stable complex, resulting in phosphorylation of type I receptors by type I I receptors with constitutive kinase activity, and subsequently the initiates activation of downstream molecules. The activin A type I IB receptor displays a 3- to 4-fold higher affinity for the ligand than the type I I receptor.

References

1. Hilden, K. et al., 1994, Blood, 83: 2163-70.
2. Persson, U. et al., 1997, J.Biol.Chem. 272: 21187-94.
3. Manova, K. et al., 1995, Mech. Dev. 1995, 49: 3-11.
4. Matzuk, MM. et al., 1992, Biochem.Biophys.Res.Commun.185: 404-13.