Cynomolgus ACVR1B / ALK-4 Protein (Cytokine)

Activin A receptor, type IB Protein Datasheet

ACVR1B / ALK-4 Protein Product Information

• Synonym: ACVR1B
• Protein Construction: A DNA sequence encoding the cynomolgus ACVR1B(F7HNS7) (Met1-Glu126) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

ACVR1B / ALK-4 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 27
• Molecular Mass: The recombinant cynomolgus ACVR1B is a disulfide-linked homodimer. The reduced monomer comprises 341 amino acids and has a calculated molecular mass of 38.2 KDa.The apparent molecular mass of it is approximately 44 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ACVR1B / ALK-4 protein

ACVR1B / ALK-4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ACVR1B / ALK-4 Protein Description

Activin receptor type-IB, also known as Serine/threonine-protein kinase receptor R2, Activin receptor-like kinase 4, ALK-4, and ACVRIB, is a single-pass type I  membrane protein which belongs to the protein kinase superfamily and TGFB receptor subfamily. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I  (I and IB) and two type I I (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with a cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. ALK-4 is expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver. ALK4 and ALK7 can mediate signal transduction by Nodal proteins, although ALK7 appears to be a receptor more specifically dedicated to Nodal signaling.

References

1. Reissmann E. et al., 2001, Genes Dev. 15 (15): 2010-22.
2. Su GH. et al., 2001, Proc Natl Acad Sci . 98 (6): 3254-7.
3. Inman GJ. et al., 2002, Mol. Pharmacol. 62 (1): 65-74.
4. Harrison CA. et al., 2003, J. Biol. Chem. 278 (23): 21129-35.