Human ACTA2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12160-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ACTA2 cDNA Clone Datasheet

ACTA2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC017554
cDNA Size:	1134
Gene Synonym:	AAT6; ACTSA; MYMY5;ACTA2
Source:	Human

ACTA2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens actin, alpha 2, smooth muscle, aorta DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ACTA2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACTA2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ACTA2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGTGAAG AAGAGGACAG CACTGCCTTG GTGTGTGACA ATGGCTCTGG GCTCTGTAAG
61       GCCGGCTTTG CTGGGGACGA TGCTCCCAGG GCTGTTTTCC CATCCATTGT GGGACGTCCC
121      AGACATCAGG GGGTGATGGT GGGAATGGGA CAAAAAGACA GCTACGTGGG TGACGAAGCA
181      CAGAGCAAAA GAGGAATCCT GACCCTGAAG TACCCGATAG AACATGGCAT CATCACCAAC
241      TGGGACGACA TGGAAAAGAT CTGGCACCAC TCTTTCTACA ATGAGCTTCG TGTTGCCCCT
301      GAAGAGCATC CCACCCTGCT CACGGAGGCA CCCCTGAACC CCAAGGCCAA CCGGGAGAAA
361      ATGACTCAAA TTATGTTTGA GACTTTCAAT GTCCCAGCCA TGTATGTGGC TATCCAGGCG
421      GTGCTGTCTC TCTATGCCTC TGGACGCACA ACTGGCATCG TGCTGGACTC TGGAGATGGT
481      GTCACCCACA ATGTCCCCAT CTATGAGGGC TATGCCTTGC CCCATGCCAT CATGCGTCTG
541      GATCTGGCTG GCCGAGATCT CACTGACTAC CTCATGAAGA TCCTGACTGA GCGTGGCTAT
601      TCCTTCGTTA CTACTGCTGA GCGTGAGATT GTCCGGGACA TCAAGGAGAA ACTGTGTTAT
661      GTAGCTCTGG ACTTTGAAAA TGAGATGGCC ACTGCCGCAT CCTCATCCTC CCTTGAGAAG
721      AGTTACGAGT TGCCTGATGG GCAAGTGATC ACCATCGGAA ATGAACGTTT CCGCTGCCCA
781      GAGACCCTGT TCCAGCCATC CTTCATCGGG ATGGAGTCTG CTGGCATCCA TGAAACCACC
841      TACAACAGCA TCATGAAGTG TGATATTGAC ATCAGGAAGG ACCTCTATGC TAACAATGTC
901      CTATCAGGGG GCACCACTAT GTACCCTGGC ATTGCCGACC GAATGCAGAA GGAGATCACG
961      GCCCTAGCAC CCAGCACCAT GAAGATCAAG ATCATTGCCC CTCCGGAGCG CAAATACTCT
1021     GTCTGGATCG GTGGCTCCAT CCTGGCCTCT CTGTCCACCT TCCAGCAGAT GTGGATCAGC
1081     AAACAGGAAT ACGATGAAGC CGGGCCTTCC ATTGTCCACC GCAAATGCTT CTAA

Quality Control:cDNA sequencing results is available upon request.