Mouse ACTA1 Gene cDNA Clone / ORF Clone

ACTA1 cDNA Clone Datasheet

ACTA1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009606.2
• cDNA Size: 1134
• Gene Synonym: Acts; Acta-2; Actsk-1; AA959943; Acta1
• Source: Mouse

ACTA1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus actin, alpha 1, skeletal muscle DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 438 T/C,735 C/T and 888 T/C not causing the amino acid variation.

ACTA1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACTA1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ACTA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGCGACG AAGACGAGAC CACCGCTCTT GTGTGTGACA ACGGCTCTGG CCTGGTGAAA
61       GCTGGCTTTG CCGGGGATGA TGCCCCCAGG GCTGTGTTCC CATCCATCGT GGGCCGACCC
121      CGTCACCAGG GTGTCATGGT AGGTATGGGT CAGAAGGACT CCTACGTGGG TGATGAGGCC
181      CAGAGCAAGC GAGGTATCCT GACCCTGAAG TACCCCATTG AACATGGCAT CATCACCAAC
241      TGGGACGACA TGGAGAAGAT CTGGCACCAC ACCTTCTACA ATGAGCTGCG TGTGGCCCCT
301      GAGGAGCACC CGACTCTGCT CACCGAGGCC CCCCTGAACC CCAAAGCTAA CCGGGAGAAG
361      ATGACTCAAA TCATGTTTGA GACCTTCAAC GTGCCTGCCA TGTATGTGGC TATCCAGGCG
421      GTGCTGTCCC TCTATGCCTC CGGCCGTACC ACCGGCATCG TGTTGGATTC TGGGGACGGT
481      GTCACCCACA ACGTGCCCAT CTATGAGGGC TATGCCCTGC CACACGCCAT CATGCGTCTG
541      GACCTGGCCG GTCGCGACCT CACTGACTAC CTGATGAAAA TCCTCACTGA GCGTGGCTAT
601      TCCTTCGTGA CCACAGCTGA ACGTGAGATT GTGCGCGACA TCAAAGAGAA GCTGTGCTAT
661      GTGGCCCTGG ACTTCGAGAA TGAGATGGCC ACCGCTGCCT CTTCCTCCTC CCTGGAGAAG
721      AGCTATGAGC TGCCTGACGG GCAGGTCATC ACCATCGGCA ATGAGCGTTT CCGTTGCCCG
781      GAGACGCTCT TCCAGCCTTC CTTTATCGGT ATGGAGTCTG CGGGGATCCA TGAGACCACC
841      TACAACAGCA TCATGAAGTG CGACATCGAC ATCAGGAAGG ACCTGTACGC CAACAACGTC
901      ATGTCAGGGG GCACCACCAT GTACCCTGGT ATCGCTGACC GCATGCAGAA GGAGATCACA
961      GCTCTGGCTC CCAGCACCAT GAAGATCAAG ATCATCGCCC CCCCTGAGCG CAAGTACTCA
1021     GTGTGGATCG GTGGCTCCAT CCTGGCCTCG CTGTCCACCT TCCAGCAGAT GTGGATCACC
1081     AAGCAGGAGT ACGACGAGGC TGGCCCCTCC ATTGTGCACC GCAAATGCTT CTAG

 

Quality Control:cDNA sequencing results is available upon request.