SP-10 Matched ELISA Antibody Pair Set,Human

Cat: SEK11789
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SP-10 Matched ELISA Antibody Pair Set,Human General Information
Product name
SP-10 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human SP-10
Assay range
46.88-3000
Materials provided
1. Capture Antibody 0.1 mg/mL of mouse anti-ACRV1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 11789-MM01)
2. Detection Antibody 0.5 mg/mL mouse anti-ACRV1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11789-MM06)
3. Standard Each vial contains 105 ng of recombinant ACRV1. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
Product overview
This SP-10 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human SP-10 . It contains Human SP-10 capture antibody, Human SP-10 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human SP-10 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
SP-10 Matched ELISA Antibody Pair Set,Human Images
Human ACRV1 ELISA Pair Set Standard Curve 13950
SP-10 Matched ELISA Antibody Pair Set,Human Alternative Names
D11S4365 Matched ELISA Antibody Pair Set, Human;SP-10 Matched ELISA Antibody Pair Set, Human;SPACA2 Matched ELISA Antibody Pair Set, Human
SP-10 Background Information

Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. ACRV1 / SP-10 is a testis-specific acrosomal protein that has been detected in several species including humans. It may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. ACRV1 / SP-10 may be involved in sperm-zona binding or penetration. It is also a intra-acrosomal protein that is considered to be a vaccine candidate for immunocontraception.

Full Name
acrosomal vesicle protein 1
References
  • Wright RM, et al.,1990, Biol Reprod. 42 (4): 693-701.
  • Kurth,B.E. et al., 1993, Anat Rec. 236 (4):619-25.
  • Golden WL, et al.,1994, Genomics.18 (2): 446-9. 
  • Foster,J.A. et al., 1994, Biol Reprod. 51 (6):1222-31.
  • Abhyankar,M.M. et al., 2007, J Biol Chem. 282 (50): 36143-54.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
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