Anti-SP-10 Antibody

Cat: 11789-MM06
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Anti-SP-10 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-SP-10 Antibody
Validated applications
ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human SP-10
No cross-reactivity with Human cell lysate (293 cell line) in ELISA.
Immunogen
Recombinant Human ACRV1 Protein (Catalog#11789-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACRV1 (rhACRV1; Catalog#11789-H08H, P26436-1, Gln 22-Ile 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #3D2F10
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SP-10 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human ACRV1 in ELISA pair set (Catalog: # SEK11789). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11789-MM01).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SP-10 Antibody Alternative Names
Anti-D11S4365 Antibody;Anti-SP-10 Antibody;Anti-SPACA2 Antibody
SP-10 Background Information

Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. ACRV1 / SP-10 is a testis-specific acrosomal protein that has been detected in several species including humans. It may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. ACRV1 / SP-10 may be involved in sperm-zona binding or penetration. It is also a intra-acrosomal protein that is considered to be a vaccine candidate for immunocontraception.

Full Name
acrosomal vesicle protein 1
References
  • Wright RM, et al.,1990, Biol Reprod. 42 (4): 693-701.
  • Kurth,B.E. et al., 1993, Anat Rec. 236 (4):619-25.
  • Golden WL, et al.,1994, Genomics.18 (2): 446-9. 
  • Foster,J.A. et al., 1994, Biol Reprod. 51 (6):1222-31.
  • Abhyankar,M.M. et al., 2007, J Biol Chem. 282 (50): 36143-54.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SP-10 Antibody Mouse Monoclonal ELISA(Cap) 11789-MM01
    Anti-SP-10 Antibody Rabbit Polyclonal ELISA 11789-RP01
    Anti-SP-10 Antibody Rabbit Polyclonal ELISA,IHC-P 11789-RP02
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