Mouse ACPP / PSAP Protein (His Tag)

Acid phosphatase, prostate Protein Datasheet

ACPP / PSAP Protein Product Information

• Synonym: 9.104100899-104272570.1, 5'-NT, A030005E02Rik, FRAP, Lap, PAP, Ppal
• Protein Construction: A DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

ACPP / PSAP Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 32
• Molecular Mass: The recombinant mouse ACPP consists of 361 amino acids and predicts a molecular mass of 42 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACPP is approximately 47 KDa as a result of glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ACPP / PSAP protein

ACPP / PSAP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ACPP / PSAP Protein Description

Prostatic acid phosphatase, also known as ACPP, is a secreted and a single-pass type I membrane protein which belongs to the histidine acid phosphatase family. ACPP / PSAP is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases. The highest levels of ACPP / PSAP are found in metastasized prostate cancer. Diseases of the bone, such as Paget's disease or hyperparathyroidism, diseases of blood cells, such as sickle-cell disease or multiple myeloma or lysosomal storage diseases, such as Gaucher's disease, will show moderately increased levels. ACPP / PSAP was used to monitor and assess progression of prostate cancer until the introduction of prostate specific antigen (PSA), which has now largely displaced it. ACPP / PSAP has a role in prognosticating intermediate and high-risk prostate cancer, it has led to renewed interest in this marker. Its physiological function may be associated with the liquefaction process of semen. ACPP / PSAP may play an important role in the transmission of HIV. ACPP / PSAP forms fibers made of amyloid. There fibers capture HIV virions promoting their attachment to target cells. The association of ACPP / PSAP with HIV may increase the ability of the virus to infect human cells "by several orders of magnitude." ACPP / PSAP may be a future target of efforts to combat the spread of HIV infection.

References

1. Genega EM. et al., 2000, Mod Pathol. 13 (11): 1186-91.
2. Taira A. et al.,2007, Oncology. 21 (8): 1003-10.
3. Münch J. et al., 2007, Cell. 131 (6): 1059-71.
4. Zylka MJ. et al.,2008, Neuron. 60 (1): 111-22.