Mouse ACO2 Gene cDNA Clone / ORF Clone

ACO2 cDNA Clone Datasheet

ACO2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_080633.2
• cDNA Size: 2343
• Gene Synonym: Aco3; Aco-2; D10Wsu183e; Aco2
• Source: Mouse

ACO2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus aconitase 2, mitochondrial DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 408 T/A,576 T/C,882 G/A and 2229 G/A not causing the amino acid variation.

ACO2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACO2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ACO2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCCTT ACAGCCTCCT GGTCACCCGG CTGCAGAAGG CCCTGGGTGT GCGGCAGTAC
61       CATGTGGCCT CTGTCCTGTG CCAACGGGCC AAGGTGGCCA TGAGCCATTT TGAGCCCAGT
121      GAGTACATCC GATATGACCT GCTAGAGAAG AACATTAACA TTGTCCGTAA ACGGTTGAAC
181      CGGCCTCTTA CTCTCTCAGA GAAGATTGTA TATGGACACC TGGATGACCC AGCCAACCAG
241      GAGATCGAGC GGGGAAAGAC ATACCTGCGT TTACGGCCCG ACCGGGTGGC CATGCAGGAT
301      GCCACAGCCC AGATGGCTAT GCTACAGTTC ATTAGCAGTG GGCTGCCTAA GGTGGCTGTA
361      CCATCAACCA TCCACTGTGA TCATCTGATT GAGGCCCAGG TTGGGGGAGA GAAAGACCTG
421      CGCAGGGCCA AGGACATAAA CCAGGAAGTG TATAATTTCC TGGCAACTGC AGGTGCCAAG
481      TATGGTGTGG GCTTCTGGAG GCCTGGATCT GGGATCATTC ACCAGATCAT TCTAGAAAAC
541      TATGCATACC CTGGAGTTCT TCTGATCGGC ACTGACTCGC ACACCCCCAA TGGTGGTGGC
601      CTGGGAGGCA TCTGCATTGG AGTAGGGGGT GCTGATGCTG TGGATGTCAT GGCTGGGATC
661      CCCTGGGAGC TGAAGTGTCC CAAGGTGATC GGTGTGAAGC TGACAGGCTC CCTCTCTGGT
721      TGGACCTCAC CCAAAGATGT GATCCTGAAA GTGGCAGGTA TCCTCACGGT GAAAGGTGGC
781      ACAGGTGCTA TTGTGGAATA CCACGGACCC GGTGTCGATT CCATCTCCTG CACTGGCATG
841      GCAACTATCT GCAACATGGG TGCAGAAATT GGGGCCACTA CATCAGTGTT CCCATACAAC
901      CACAGGATGA AAAAGTACCT GAGCAAGACA GGCCGAACAG ACATTGCCAA CCTAGCAGAA
961      GAATTCAAGG ATCACTTAGT GCCTGATCCT GGCTGCCAGT ATGACCAAGT GATTGAAATT
1021     AACCTCAATG AGCTAAAGCC ACATATCAAT GGGCCCTTTA CCCCTGACTT GGCTCACCCA
1081     GTGGCAGATG TGGGGACTGT GGCAGAGAAG GAAGGCTGGC CTCTGGACAT CAGAGTAGGT
1141     TTGATTGGCA GCTGCACCAA TTCAAGCTAT GAGGACATGG GACGATCAGC AGCTGTGGCT
1201     AAGCAGGCAT TGGCCCACGG ACTCAAGTGC AAGTCCCAGT TCACCATCAC CCCAGGCTCT
1261     GAGCAGATCC GTGCCACTAT TGAGCGGGAT GGCTATGCAC AGATCTTAAG GGATGTGGGT
1321     GGAATCGTTT TGGCCAATGC CTGTGGCCCT TGCATCGGCC AGTGGGACAG AAAAGACATC
1381     AAGAAGGGGG AGAAGAATAC AATCGTCACC TCTTACAACA GGAACTTCAC AGGCCGTAAT
1441     GATGCAAACC CTGAGACCCA TGCCTTTGTC ACATCCCCAG AGATTGTCAC AGCTCTGGCC
1501     ATTGCAGGAA CCCTGAAGTT CAATCCAGAA ACTGACTTCT TAACAGGCAA GGACGGCAAG
1561     AAGTTCAAGT TGGAGGCTCC AGATGCAGAC GAGCTTCCCC GATCGGACTT TGATCCAGGG
1621     CAGGACACAT ACCAGCACCC CCCTAAGGAC AGCAGTGGGC AGCGGGTGGA CGTGAGCCCT
1681     ACCAGCCAGC GCCTGCAGCT CCTGGAGCCT TTCGACAAGT GGGACGGCAA AGACCTCGAG
1741     GACCTGCAAA TCCTCATCAA GGTCAAAGGG AAGTGCACCA CAGACCACAT TTCTGCTGCC
1801     GGCCCCTGGC TCAAGTTCCG TGGGCATCTG GATAACATCT CTAACAACCT GCTCATCGGT
1861     GCCATCAACA TCGAAAATGG CAAAGCCAAC TCTGTACGCA ATGCTGTCAC CCAGGAGTTT
1921     GGCCCTGTAC CTGACACTGC TCGCTACTAC AAGAAACATG GCATCAGGTG GGTGGTGATT
1981     GGAGATGAGA ACTATGGTGA GGGCTCAAGC CGGGAGCATG CAGCACTGGA GCCTCGCCAC
2041     CTTGGGGGCC GGGCCATCAT CACCAAGAGC TTCGCCAGGA TCCACGAAAC CAATCTAAAG
2101     AAGCAGGGCT TGCTGCCCCT CACCTTCGCT GACCCCTCCG ACTATAACAA GATTCATCCA
2161     GTGGACAAGC TGACCATTCA AGGCCTGAAG GACTTTGCCC CTGGAAAGCC TCTGAAGTGC
2221     GTCATCAAAC ACCCCAATGG GACCCAGGAG ACCATCCTCC TGAACCACAC CTTCAACGAG
2281     ACCCAGATCG AGTGGTTCCG TGCAGGCAGT GCCCTCAACA GGATGAAGGA GCTGCAGCAG
2341     TGA

 

Quality Control:cDNA sequencing results is available upon request.