ACO1 / irp1 Protein (His Tag)

Aconitase 1, soluble Protein Datasheet

ACO1 / irp1 Protein Product Information

• Synonym: RP11-334P12.1, ACONS, IREB1, IREBP, IREBP1, IRP1
• Protein Construction: A DNA sequence encoding the human ACO1 (P21399?) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

ACO1 / irp1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human ACO1 consists of 908 amino acids and has a calculated molecular mass of 101 KDa. It migrates as an approximately 90 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0, 10% gly, 2mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ACO1 / irp1 protein

ACO1 / irp1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ACO1 / irp1 Protein Description

Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase 1(ACO1) or IRP1 contains four domains; the first three from the N-terminus are closely associated around the Fe-S cluster. Association of the larger C-terminal domain with the first three domains createsan extensive cleft leading to the Fe-S cluster. Residues from all four domains contribute to the active site region. Aconitase 1(ACO1) or IRP1 can assume two different functions in cells, depending o n different conditions. During iron scarcity or oxidative stress, Aconitase 1(ACO1) or IRP1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, Aconitase 1(ACO1) or IRP1 binds an iron-sulfur cluster to function as a cytosolic aconitase.

References

1. Robbins AH. et al., 1989, Proteins Structure Function and Bioinformatics. 5 (4): 289-312
2. Volz K. 2008, Curr Opin Struct Biol. 18 (1):106-11.
3. Gruer MJ. et al., 1997, Trends Biochem Sci. 22 (1): 3-6.