ACO1 / irp1 Protein Price Inquiry ( Available Sizes )
ACO1 / irp1 Protein Product Information
||RP11-334P12.1, ACONS, IREB1, IREBP, IREBP1, IRP1
||A DNA sequence encoding the human ACO1 (P21399?) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.
ACO1 / irp1 Protein QC Testing
||> 95 % as determined by SDS-PAGE.
ACO1 / irp1 protein
||< 1.0 EU per μg of the protein as determined by the LAL method
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
||The recombinant human ACO1 consists of 908 amino acids and has a calculated molecular mass of 101 KDa. It migrates as an approximately 90 KDa band in SDS-PAGE under reducing conditions.
||Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0, 10% gly, 2mM DTT
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
ACO1 / irp1 Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
ACO1 / irp1 Protein Related Products & Topics
ACO1 / irp1 Protein Description
Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase 1(ACO1) or IRP1 contains four domains; the first three from the N-terminus are closely associated around the Fe-S cluster. Association of the larger C-terminal domain with the first three domains createsan extensive cleft leading to the Fe-S cluster. Residues from all four domains contribute to the active site region. Aconitase 1(ACO1) or IRP1 can assume two different functions in cells, depending o n different conditions. During iron scarcity or oxidative stress, Aconitase 1(ACO1) or IRP1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, Aconitase 1(ACO1) or IRP1 binds an iron-sulfur cluster to function as a cytosolic aconitase.
- Robbins AH. et al., 1989, Proteins Structure Function and Bioinformatics. 5 (4): 289-312
- Volz K. 2008, Curr Opin Struct Biol. 18 (1):106-11.
- Gruer MJ. et al., 1997, Trends Biochem Sci. 22 (1): 3-6.