Cynomolgus monkey ACE2 Gene cDNA Clone / ORF Clone

ACE2 cDNA Clone Datasheet

ACE2 cDNA Clone Product Information

• cDNA Size: 2418
• Gene Synonym: ACE2
• Source: Cynomolgus monkey

ACE2 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1397 G/A resulting in the amino acid Gly substitution by Asp.
  Please check the sequence information before order.

ACE2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACE2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ACE2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCAGGCT CTTCCTGGCT CCTTCTCAGC CTTGTTGCTG TAACTGCTGC TCAGTCCACC
61       ATTGAGGAAC AGGCCAAGAC ATTTTTGGAC AAGTTTAACC ACGAAGCCGA AGACCTGTTC
121      TATCAAAGTT CACTTGCTTC TTGGAATTAT AACACCAATA TTACTGAAGA GAATGTCCAA
181      AACATGAATA ATGCTGGGGA AAAATGGTCT GCCTTTTTAA AAGAACAGTC CACACTTGCC
241      CAAATGTATC CACTGCAAGA AATTCAGAAT CTCACAGTCA AGCTTCAGTT GCAGGCTCTT
301      CAGCAAAATG GGTCTTCAGT GCTCTCAGAA GACAAGAGCA AACGGTTGAA CACAATTCTA
361      AATACAATGA GCACCATCTA CAGTACTGGA AAAGTTTGTA ACCCAAATAA TCCCCAGGAA
421      TGCTTATTAC TTGATCCAGG TTTGAATGAA ATAATGGAAA AGAGTTTAGA CTACAATGAG
481      AGGCTCTGGG CTTGGGAAGG CTGGAGATCT GAGGTCGGCA AGCAGCTGAG GCCATTATAT
541      GAAGAGTATG TGGTCTTGAA AAATGAGATG GCAAGAGCAA ATCATTATAA GGACTATGGG
601      GATTATTGGA GAGGAGACTA TGAAGTAAAC GGGGTAGATG GCTATGACTA CAACCGCGAC
661      CAGTTGATTG AAGATGTGGA ACGTACCTTT GAAGAGATTA AACCATTATA TGAACATCTT
721      CATGCCTATG TGAGGGCAAA GTTGATGAAT GCCTACCCTT CCTATATTAG TCCAACTGGA
781      TGCCTTCCTG CTCATTTGCT TGGTGATATG TGGGGTAGAT TTTGGACAAA TCTGTACTCT
841      TTGACAGTTC CCTTTGGACA GAAACCAAAC ATAGATGTTA CTGATGCAAT GGTGAACCAG
901      GCCTGGAATG CACAGAGAAT ATTCAAGGAG GCCGAGAAGT TCTTTGTATC TGTTGGTCTT
961      CCTAATATGA CTCAAGGATT CTGGGAAAAT TCCATGCTAA CTGATCCAGG AAATGTTCAG
1021     AAAGTAGTCT GCCACCCCAC AGCTTGGGAC CTGGGGAAGG GTGACTTCAG GATCATTATG
1081     TGCACAAAGG TGACAATGGA CGACTTCCTG ACAGCTCATC ATGAGATGGG GCATATCCAA
1141     TATGATATGG CATATGCTGC ACAACCTTTT CTGCTAAGAA ATGGAGCTAA TGAAGGATTC
1201     CATGAAGCTG TTGGGGAAAT CATGTCACTT TCTGCAGCCA CACCTAAGCA TTTAAAATCC
1261     ATTGGTCTTC TGTCACCTGA TTTTCAAGAA GACAATGAAA CAGAAATAAA CTTCCTGCTC
1321     AAACAAGCAC TCACGATTGT TGGGACTCTG CCATTTACTT ACATGTTAGA GAAGTGGAGG
1381     TGGATGGTCT TTAAAGATGA AATTCCCAAA GATCAGTGGA TGAAAAAGTG GTGGGAGATG
1441     AAGCGAGAGA TAGTTGGGGT GGTGGAACCT GTGCCCCATG ATGAAACATA CTGTGACCCC
1501     GCATCTCTGT TCCATGTTTC TAATGATTAC TCATTCATTC GATATTACAC AAGGACCCTT
1561     TACCAATTCC AGTTTCAAGA AGCACTTTGT CAAGCAGCTA AACACGAAGG CCCTCTGCAC
1621     AAATGTGACA TCTCAAACTC TACAGAAGCT GGACAGAAAT TGCTCAATAT GCTGAAACTT
1681     GGAAAATCAG AACCCTGGAC CCTAGCATTG GAAAATGTTG TAGGAGCAAA GAACATGAAT
1741     GTAAGACCAC TGCTCAACTA CTTTGAGCCC TTGTTTACCT GGCTGAAAGA CCAGAACAAG
1801     AATTCTTTTG TGGGATGGAG TACCGACTGG AGTCCGTATG CTGACCAAAG CATCAAAGTG
1861     AGGATAAGCC TAAAATCAGC TCTTGGAGAT AAAGCATATG AATGGAACGA CAATGAAATG
1921     TACCTGTTCC GATCATCTGT TGCATATGCC ATGAGGACGT ACTTTTTAGA AATCAAACAT
1981     CAGACGATTC TTTTTGGGGA GGAGGATGTG CGAGTTGCTG ATTTGAAACC AAGAATCTCC
2041     TTTAATTTCT ATGTCACTGC ACCTAAAAAT GTGTCTGACA TCATTCCTAG AACTGAAGTT
2101     GAAGAGGCCA TCAGGATCTC CAGGAGCCGT ATCAATGATG CTTTCCGTCT GAATGACAAC
2161     AGCCTGGAGT TTCTGGGGAT ACAGACAACA CTTGCACCTC CTTACCAGTC CCCCGTTACC
2221     ACATGGCTAA TTGTTTTTGG AGTTGTGATG GGAGTGATAG TGGCTGGCAT TGTCGTCCTG
2281     ATCTTCACTG GGATCAGAGA TCGAAAGAAG AAAAATCAAG CAAGAAGTGA AGAGAATCCT
2341     TATGCCTCCA TCGATATTAA CAAAGGAGAA AATAATCCAG GATTCCAAAA CACTGATGAT
2401     GTTCAGACCT CCTTTTAG

 

Quality Control:cDNA sequencing results is available upon request.