+86-400-890-9989
Product Catalog
Research Topics
Rat ACE Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80406-G |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
ACE cDNA Clone Datasheet
ACE cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_012544.1 |
|
cDNA Size: |
3942 |
| Gene Synonym: |
Dcp1; StsRR92; Ace |
| Source: | Rat |
ACE cDNA Clone Description
|
cDNA Description: |
ORF Clone of Rattus norvegicus angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutations: 529 G/A，620 A/G resulting in the amino acid Glu substitution by Lys, Lys substitution by Arg and 2649 T/C，2940 G/A not causing the amino acid variation. |
ACE cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
ACE cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
ACE cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGGGCCG CGTCCGGCCA GCGGGGGCGG TGGCCGTTGT CACCGCCGCT CTTGATGCTG 61 TCGCTGCTGC TGCTGCTGCT GCTGCCGCCG TCGCCCGCCC CGGCGCTTGA CCCTGGATTG 121 CAGCCGGGCA ACTTTTCCGC GGACGAGGCA GGGGCGCAGC TCTTCGCTGA CAGCTATAAC 181 TCGAGTGCCG AGGTGGTGAT GTTCCAGAGC ACCGCAGCCA GCTGGGCGCA CGACACCAAC 241 ATCACGGAGG AGAATGCGCG GCTCCAGGAG GAAGCGGCCC TGATCAACCA GGAGTTTGCA 301 GAGGTCTGGG GCAAGAAGGC CAAGGAGCTG TATGAGTCCA TCTGGCAGAA CTTCACTGAC 361 CAAAAGCTGC GAAGGATCAT CGGATCCGTA CAGACCCTAG GACCTGCCAA CCTGCCCCTG 421 ACCCAGCGGC TGCAGTACAA CTCTCTGCTA AGCAACATGA GCAGAATCTA CTCCACCGGC 481 AAGGTCTGCT TCCCCAACAA GACTGCCACC TGCTGGTCCC TGGACCCAAA GCTCACCAAC 541 ATCCTGGCTT CCTCACGAAA CTATGCCAAG GTGCTGTTTG CCTGGGAAGG CTGGCATGAT 601 GCTGTGGGTA TCCCACTGAG GCCCCTCTAT CAGGACTTTA CTGCCCTCAG TAATGAAGCC 661 TACAGACAAG ATGGCTTCTC AGACACAGGA GCCTACTGGC GCTCCTGGTA TGAGTCCCCC 721 TCCTTTGAAG AGAGTTTGGA GCATCTCTAC CACCAAGTCG AGCCCCTCTA CCTGAACCTC 781 CATGCCTTTG TCCGTCGCGC ACTGCACCGC CGCTATGGGG ACAAATACAT CAATCTCAGA 841 GGTCCTATTC CCGCTCATCT GCTGGGAGAC ATGTGGGCGC AGAGCTGGGA GAACATTTAC 901 GACATGGTAG TGCCTTTCCC GGACAAACCC AACCTCGATG TCACCAGTAC AATGGTACAG 961 AAGGGCTGGA ATGCCACGCA CATGTTCCGG GTCGCAGAGG AATTCTTTAC CTCGCTGGGG 1021 CTCTCCCCCA TGCCTCCAGA GTTCTGGGCG GAGTCGATGC TGGAGAAACC AGCTGATGGA 1081 CGGGAGGTGG TGTGCCATGC CTCTGCGTGG GACTTCTACA ACAGGAAGGA CTTCAGGATT 1141 AAGCAGTGCA CGCGGGTCAC GATGGACCAG CTGTCCACAG TACACCACGA GATGGGCCAC 1201 GTGCAGTACT ATCTCCAGTA CAAGGACCTG CACGTCTCTC TGCGTCGAGG TGCCAACCCT 1261 GGCTTCCACG AGGCCATCGG GGATGTACTC GCTCTCTCTG TCTCTACCCC AGCACATCTG 1321 CACAAAATTG GCCTGCTAGA CCGTGTTGCC AATGACATAG AAAGTGACAT CAATTACTTG 1381 CTAAAGATGG CCCTAGAGAA AATTGCCTTC TTGCCCTTTG GTTACCTGGT GGACCAGTGG 1441 CGCTGGGGGG TCTTCAGTGG ACGTACCCCA CCCTCTCGCT ACAACTACGA CTGGTGGTAT 1501 CTTCGAACCA AGTATCAGGG GATCTGCCCA CCAGTTGCTC GGAATGAAAC CCATTTTGAC 1561 GCTGGGGCCA AGTTTCACAT CCCAAGCGTG ACACCATACA TCAGGTACTT TGTGAGTTTC 1621 GTGCTACAGT TCCAGTTCCA TCAAGCGCTG TGCAAGGAGG CAGGCCACCA GGGTCCACTA 1681 CACCAGTGTG ACATCTACCA GTCCACCAAG GCAGGGGCCA AGCTCCAACA GGTGCTGCAG 1741 GCTGGCTGCT CCAGGCCCTG GCAGGAGGTG CTGAAGGACC TGGTGGGTTC AGATGCGCTG 1801 GATGCCAGTG CGCTAATGGA GTACTTCCAA CCAGTAAGCC AGTGGCTGCA GGAGCAGAAT 1861 CAGCGGAATG GCGAGGTCCT AGGCTGGCCG GAGTATCAGT GGCGTCCACC GTTACCAGAC 1921 AACTATCCAG AGGGAATTGA CCTAGAGACT GATGAAGCCA AGGCTAACAG GTTCGTGGAG 1981 GAGTATGACC GGACAGCCAA GGTGTTGTGG AACGAATACG CAGAGGCCAA CTGGCATTAT 2041 AACACCAACA TTACCATAGA GGGCAGCAAG ATCCTGCTTC AGAAAAACAA GGAAGTGTCC 2101 AACCATACCT TGAAATATGG CACCTGGGCC AAGACATTTG ACGTGAGCAA CTTCCAGAAC 2161 TCTACCATCA AGCGGATCAT AAAGAAGGTT CAGAACGTGG ACCGGGCAGT GCTGCCTCCC 2221 AACGAGTTAG AAGAGTACAA CCAGATCCTG CTAGACATGG AGACGACTTA CAGTGTAGCC 2281 AATGTTTGCT ACACAAATGG CACTTGTCTG TCACTGGAGC CTGATCTGAC AAATATAATG 2341 GCCACGTCCC GGAAATACGA AGAATTGCTT TGGGTGTGGA AGAGCTGGCG AGACAAGGTG 2401 GGGAGAGCCA TCCTTCCCTT TTTCCCAAAG TACGTGGACT TCTCCAACAA GATCGCCAAG 2461 CTCAACGGCT ACTCTGATGC AGGGGATTCC TGGAGATCCT CATATGAGTC CGACGACTTG 2521 GAGCAAGACC TGGAAAAACT ATACCAGGAG CTGCAGCCGC TCTACCTGAA CCTGCATGCC 2581 TATGTGCGCC GCTCCCTGCA CCGCCATTAT GGGTCTGAGT ACATCAACCT GGATGGTCCC 2641 ATTCCTGCCC ACCTGCTAGG GAACATGTGG GCACAGACTT GGTCCAACAT CTATGACTTG 2701 GTGGCACCCT TCCCTTCCGC CCCCAGTATA GATGCCACGG AGGCCATGAT AAAGCAGGGA 2761 TGGACACCCA GAAGGATATT TAAGGAAGCT GACAATTTTT TTACCTCCCT GGGGCTGTTA 2821 CCTGTGCCCC CTGAGTTCTG GAACAAGTCA ATGTTAGAGA AGCCAACCGA TGGGAGGGAG 2881 GTGGTGTGCC ATGCCTCAGC CTGGGACTTC TACAACGGCA AGGACTTCAG GATCAAGCAA 2941 TGTACCTCTG TGAACATGGA GGAATTGGTG ATAGCCCACC ACGAAATGGG CCACATCCAG 3001 TATTTCATGC AGTACAAAGA CTTGCCTGTG ACCTTTCGGG AGGGCGCCAA CCCCGGTTTT 3061 CATGAGGCTA TTGGAGATGT TTTGGCTCTG TCTGTGTCTA CACCCAAGCA TCTACACAGT 3121 CTCAACCTGC TCAGCAGTGA GGGCAGTGGC TACGAGCATG ACATCAACTT TCTAATGAAG 3181 ATGGCCCTTG ACAAGATCGC CTTCATCCCC TTCAGCTACC TCATTGACCA GTGGCGCTGG 3241 AGGGTCTTTG ACGGAAGCAT CACCAAGGAG AACTACAACC AGGAGTGGTG GAGTCTCAGA 3301 CTGAAGTACC AGGGTCTCTG CCCTCCAGTG CCTAGATCCC AAGGTGACTT TGACCCAGGG 3361 TCCAAGTTCC ACGTTCCTGC GAATGTGCCA TACATCAGGT ACTTTATCAG CTTCATCATC 3421 CAGTTCCAGT TCCACGAGGC ACTATGTCGC GCAGCCGGGC ACACCGGCCC CCTGTACAAG 3481 TGTGATATCT ACCAATCCAA GGAAGCAGGG AAGCTGCTGG CAGATGCCAT GAAGTTGGGC 3541 TACAGTAAGC AGTGGCCAGA AGCCATGAAG ATAATCACAG GCCAACCTAA CATGTCAGCC 3601 TCTGCCATTA TGAATTACTT CAAGCCACTG ACTGAATGGC TCGTCACAGA GAACAGGAGA 3661 CATGGAGAGA CACTGGGCTG GCCGGAGTAC ACCTGGACAC CAAACACGGC TCGTGCAGAA 3721 GGCTCCCTCC CAGAGTCCAG TCGCGTCAAC TTCCTGGGTA TGTACCTGGA ACCACAGCAG 3781 GCCCGTGTGG GCCAGTGGGT GCTGCTCTTC CTAGGCGTCG CCCTGCTGGT GGCCACCGTG 3841 GGTCTCGCCC ACCGACTCTA CAACATCCAT AACCATCACA GCCTCCGCCG GCCCCACCGT 3901 GGGCCCCAGT TTGGGTCCGA GGTGGAGCTC AGACACTCCT GA
Quality Control:cDNA sequencing results is available upon request.
