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Human ACE Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG11598-M |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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ACE cDNA Clone Datasheet
ACE cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_000789.2 |
|
cDNA Size: |
3921 |
| Gene Synonym: |
DCP; ACE1; DCP1; CD143; MGC26566; ACE |
| Source: | Human |
ACE cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 DNA. |
| Vector: | Cloning Vector: pMD18-T Simple Vector.
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: |
Identical with the Gene Bank Ref. ID sequence except for five point mutations: 1215 C/T, 2193 A/G, 2328 G/A and 3387 T/C not causing the amino acid variation and 3853 C/T resulting in the amino acid His substitution by Tyr. |
ACE cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
ACE cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.
ACE cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG 61 CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT 121 GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG 181 CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA 241 AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG 301 GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC 361 ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC 421 AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC 481 AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA 541 AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG 601 AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC 661 ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG 721 GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC 781 GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT 841 CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC 901 CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG 961 CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC 1021 GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC 1081 GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC 1141 ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG 1201 TACAAGGATC TGCCTGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT 1261 GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG 1321 GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA 1381 AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT 1441 GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG 1501 GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT 1561 GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC 1621 CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC 1681 CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC 1741 TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC 1801 AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC 1861 CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA 1921 GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC 1981 CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA 2041 GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC 2101 GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC 2161 ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCGCTGCCTG CCCAGGAGCT GGAGGAGTAC 2221 AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT 2281 GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACATC CCGGAAATAT 2341 GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG 2401 TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT 2461 GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG 2521 CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG 2581 CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG 2641 GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA 2701 GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG 2761 TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC 2821 TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG 2881 GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG 2941 GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA 3001 GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC 3061 GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT 3121 GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC 3181 GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC 3241 ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC 3301 TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT 3361 TCTAGCGTGC CTTACATCAG GTACTTCGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG 3421 GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC 3481 AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG 3541 GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC 3601 TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC 3661 TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC 3721 GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG 3781 CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC 3841 TTCAGCATCC GCTACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG 3901 GTGGAGCTGA GACACTCCTG A
Quality Control:cDNA sequencing results is available upon request.
