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Human ACE Gene cDNA Clone (full-length ORF Clone)
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Human ACE Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG11598-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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ACE cDNA Clone Datasheet

 

ACE cDNA Clone Product Information

Gene Bank Ref.ID: NM_000789.2

cDNA Size:

3921

Gene Synonym:

DCP; ACE1; DCP1; CD143; MGC26566; ACE

Source: Human

ACE cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description:

Identical with the Gene Bank Ref. ID sequence except for five point mutations: 1215 C/T, 2193 A/G, 2328 G/A and 3387 T/C not causing the amino acid variation and 3853 C/T resulting in the amino acid His substitution by Tyr.

ACE cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACE cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ACE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG
61       CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
121      GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
181      CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
241      AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
301      GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
361      ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
421      AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
481      AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
541      AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
601      AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
661      ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
721      GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
781      GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
841      CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
901      CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
961      CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
1021     GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
1081     GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
1141     ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
1201     TACAAGGATC TGCCTGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
1261     GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
1321     GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
1381     AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
1441     GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
1501     GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
1561     GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
1621     CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
1681     CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
1741     TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
1801     AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
1861     CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
1921     GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
1981     CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
2041     GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
2101     GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
2161     ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCGCTGCCTG CCCAGGAGCT GGAGGAGTAC
2221     AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
2281     GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACATC CCGGAAATAT
2341     GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
2401     TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
2461     GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
2521     CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
2581     CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
2641     GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
2701     GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
2761     TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
2821     TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
2881     GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
2941     GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
3001     GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
3061     GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
3121     GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
3181     GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
3241     ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
3301     TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
3361     TCTAGCGTGC CTTACATCAG GTACTTCGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
3421     GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
3481     AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
3541     GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
3601     TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
3661     TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
3721     GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
3781     CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
3841     TTCAGCATCC GCTACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
3901     GTGGAGCTGA GACACTCCTG A

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"