|IP||1-2 μL/mg of lysate|
|ELISA(Det)||1:5000-1:50000||This antibody will detect Human ACBD6 in ELISA pair set (Catalog: # SEK11112). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11112-MM07).|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
ACBD6 was immunoprecipitated using:
Lane A:0.5 mg hela Whole Cell Lysate0.5 µL anti-ACBD6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-ACBD6 mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 31 kDa
Observed band size: 31 kDa
Anti-ACBD6 mouse monoclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:31 kDa
Observed band size:35 kDa
Acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32 kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and in CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.