Mouse ABHD4 Gene cDNA Clone / ORF Clone

ABHD4 cDNA Clone Datasheet

ABHD4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_134076.1
• cDNA Size: 1029
• Gene Synonym: Abh4; AI429574; 1110035H23Rik; Abhd4
• Source: Mouse

ABHD4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus abhydrolase domain containing 4 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations:376 G/A resulting in the amino acid ala substitution by thr, 915 G/A not causing the amino acid variation.

ABHD4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ABHD4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ABHD4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGATG ATCTGGAGCA GCAGCCTCAG GGCTGGCTGA GTAGCTGGCT CCCCACTTGG
61       CGCCCCACTT CCATGTCTCA ACTGAAGAAT GTGGAAGCCA GGATCCTCCA GTGTCTCCAG
121      AACAAGTTCC TGGCCCGTTA TGTATCCCTC CCAAACCAGA ACAAGATCTG GACGGTGACT
181      GTGAGCCCAG AACAAAAGGA TCGCACCCCT CTGGTGATGG TACATGGCTT TGGGGGCGGT
241      GTGGGCCTCT GGATCCTCAA CATGGATTCA CTGAGTGCCC GCCGCACACT TCATACCTTT
301      GATCTGCTTG GTTTTGGGCG AAGCTCAAGG CCAACATTCC CAAGGGACCC AGAAGGAGCT
361      GAAGATGAGT TTGTGACCTC AATAGAGACA TGGCGGGAGA CCATGGGAAT CCCCACCATG
421      ATCCTCCTGG GGCACAGTTT GGGAGGATTC CTGGCCACTT CTTACTCTAT CAAGTACCCT
481      GAAAGAGTTA AACATCTTAT CCTGGTGGAT CCATGGGGCT TTCCCCTACG ACCAACTGAC
541      CCTAGTGAGA TCCGTGCACC TCCAACCTGG GTCAAGGCTG TGGCATCTGT CCTGGGACGT
601      TCCAATCCAC TGGCTGTTCT TCGAGTGGCT GGGCCTTGGG GGCCTGGGCT GGTGCAGAGA
661      TTCCGTCCAG ACTTCAAGCG CAAGTTTGCA GACTTCTTTG AGGATGACAC CATCTCGGAA
721      TACATCTACC ACTGCAATGC ACAGAATCCC AGTGGGGAAA CGGCATTCAA AGCCATGATG
781      GAGTCCTTTG GCTGGGCCCG GCGCCCCATG TTGGAGCGAA TCCACTTAAT TCGAAAAGAT
841      GTGCCCATCA CCATGATCTA TGGGGCCAAC ACCTGGATAG ATACCAGCAC AGGGAAGAAG
901      GTGAAGATGC AAAGACCGGA TTCCTACGTC CGAGACATGG AGATCGAGGG CGCATCCCAC
961      CACGTCTATG CTGACCAGCC ACACATCTTC AATGCTGTGG TAGAAGAGAT CTGCAACTCA
1021     GTTGACTGA

 

Quality Control:cDNA sequencing results is available upon request.