ABHD4 Protein

Abhydrolase Domain-Containing Protein 4 / ABHD4

ABHD4 Protein Product Information

• Synonym: ABHD4, ABH4, FLJ12816
• Protein Construction: A DNA sequence encoding the full length of human ABHD4 ( NP_071343.2 ) ( Met 1 - Asp 342 ) was expressed, with a polyhistidine tag at the N-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

ABHD4 Protein QC Testing

• Purity: > 82 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human ABHD4 consists of 360 amino acids and predicts a molecular mass of 41 kDa. It migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl pH 8.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ABHD4 protein

ABHD4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ABHD4 Protein Description

Abhydrolase domain-containing protein 4, also known as Lyso-N-acylphosphatidylethanolamine lipase, Alpha/beta-hydrolase 4 and ABHD4, is a protein which belongs to the peptidase S33 family and ABHD4 / ABHD5 subfamily. The alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The core of each enzyme is an alpha/beta-sheet (rather than a barrel), containing 8 strands connected by helices. The enzymes are believed to have diverged from a common ancestor, preserving the arrangement of the catalytic residues. All have a catalytic triad, the elements of which are borne on loops, which are the best-conserved structural features of the fold. ABHD4 lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). ABHD4 contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. ABHD4 hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains.

References

1. Ollis, D L. et al., 1992, Protein Eng. 5 (3): 197-211.
2. Liu,J. et al., 2008, Neuropharmacology. 54 (1):1-7.
3. Katz-Jaffe,M.G. et al., 2009,Theriogenology.71 (6): 939-46.