ABHD4 Antibody (Antigen Affinity Purified)

Abhydrolase Domain-Containing Protein 4 Antibody ( Antigen Affinity Purified )

ABHD4 Antibody Product Information

• Immunogen: Recombinant human ABHD4 protein ( Catalog#11097-H07B )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human ABHD4 ( rh ABHD4 ; Catalog#11097-H07B ; NP_071343.2 ; Met 1 - Asp 342 ). ABHD4 specific IgG was purified by human ABHD4 affinity chromatography

ABHD4 Antibody Usage Guide

• Specificity: Human ABHD4
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human ABHD4 in WB. Using a DAB detection system, the detection limit for human ABHD4 is approximately 1 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human ABHD4. The detection limit for human ABHD4 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ABHD4 Antibody Background

Abhydrolase domain-containing protein 4, also known as Lyso-N-acylphosphatidylethanolamine lipase, Alpha/beta-hydrolase 4 and ABHD4, is a protein which belongs to the peptidase S33 family and ABHD4 / ABHD5 subfamily. The alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The core of each enzyme is an alpha/beta-sheet (rather than a barrel), containing 8 strands connected by helices. The enzymes are believed to have diverged from a common ancestor, preserving the arrangement of the catalytic residues. All have a catalytic triad, the elements of which are borne on loops, which are the best-conserved structural features of the fold. ABHD4 lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). ABHD4 contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. ABHD4 hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains.

References

1. Ollis, D L. et al., 1992, Protein Eng. 5 (3): 197-211.
2. Liu,J. et al., 2008, Neuropharmacology. 54 (1):1-7.
3. Katz-Jaffe,M.G. et al., 2009,Theriogenology.71 (6): 939-46.