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ABCB5

ABCB5 Protein & Antibody

Entrez Gene Summary for ABCB5


OMIM-Description for ABCB5


By searching a database for homologs of ABCB1 (171050), followed by RT-PCR of RNA from human primary epidermal melanocytes and a malignant melanoma cell line, Frank et al. (2003) cloned ABCB5. The deduced 812-amino acid protein has 5 transmembrane helices flanked by both extracellular and intracellular ATP-binding domains. ABCB5 shares 54% and 56% amino acid identity with ABCB1 and ABCB4 (171060), respectively. RT-PCR detected ABCB5 in melanocytes and melanoma cells, but not in peripheral blood mononuclear cells or nonmelanoma tumor cell lines. Western blot analysis revealed an 89-kD endogenous ABCB5 protein in melanocytes and melanoma cells. Flow cytometry showed ABCB5 expressed on the cell surface of transfected breast cancer cells. By screening a melanoma cDNA library, Chen et al. (2005) cloned 2 ABCB5 splice variants, which they called ABCB5-alpha and -beta. ABCB5-alpha and -beta diverge after exon 6, with ABCB5-alpha including a seventh exon that encodes its 3-prime UTR, and ABCB5-beta including 14 more exons. The 131-amino acid ABCB5-alpha protein has a calculated molecular mass of 15 kD. It has an ABC signature motif and a Walker B consensus sequence, but no Walker A consensus sequence. In contrast, ABCB5-beta has an N-terminal ABC signature motif and Walker B motif, followed by 6 transmembrane domains and C-terminal Walker A, ABC signature, and Walker B motifs. RT-PCR detected preferential expression of both ABCB5-alpha and -beta in melanomas, with no expression in normal uterus, lung, or placenta. Northern blot analysis detected ABCB5 transcripts of 2.4 to 7.5 kb in melanoma cells, but not in any normal human tissues examined. RT-PCR showed expression of ABCB5-alpha and -beta in normal melanocytes and of ABCB5-beta in retinal pigment epithelial cells.

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ATP-binding cassette sub-family B member 5 also known as P-glycoprotein ABCB5 is a plasma membrane-spanning protein that in humans is encoded by the ABCB5 gene. ABCB5 is an ABC transporter and P-glycoprotein family member principally expressed in physiological skin and human malignant melanoma.

ABCB5 Alternative Names

ABCB5beta,EST422562,ABCB5alpha, [homo-sapiens]
9230106F14Rik, [mus-musculus]

ABCB5 Related Products

ABCB5 Proteins

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ABCB5 Antibodies

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ABCB5 Antibody, Rabbit PAb, Antigen Affinity Purified

ABCB5 cDNA Clones

Product nameProduct name
Human ABCB5 Gene ORF cDNA clone expression plasmid, N-HA tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, C-HA tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, C-His tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, N-Flag tagHuman ABCB5 Gene ORF cDNA clone in cloning vectorHuman ABCB5 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, C-Flag tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, C-Myc tagHuman ABCB5 Gene ORF cDNA clone expression plasmidHuman ABCB5 Gene ORF cDNA clone expression plasmid, N-Myc tagHuman ABCB5 Gene ORF cDNA clone expression plasmid, N-His tag

ABCB5 ELISA

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