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AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified

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Mouse AARS Antibody Product Information
Immunogen:Recombinant Mouse AARS / alanyl-tRNA synthetase Protein (Catalog#50384-M08B)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:Produced in rabbits immunized with purified, recombinant Mouse AARS / alanyl-tRNA synthetase (rM AARS / alanyl-tRNA synthetase; Catalog#50384-M08B; Q8BGQ7; Met1-Asn968). AARS / alanyl-tRNA synthetase specific IgG was purified by Mouse AARS / alanyl-tRNA synthetase affinity chromatography.
Mouse AARS Antibody WB Application Image
Mouse AARS Antibody IHC Application Image
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Caption:
Immunochemical staining of mouse AARS in mouse kidney with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Caption:
Immunochemical staining of mouse AARS in mouse stomach with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Mouse AARS Antibody IF Application Image
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified
[Click to enlarge image]
Caption:
Immunofluorescence staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Mouse AARS Antibody IP Application Image
Other AARS Antibody Products
AARS / alanyl-tRNA synthetase Background

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

Mouse AARS / alanyl-tRNA synthetase References
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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