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Mouse AAMP Gene ORF cDNA clone in cloning vector

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    Mouse AAMP cDNA Clone Product Information
    NCBI RefSeq:NM_146110.2
    RefSeq ORF Size:1308
    cDNA Description:ORF Clone of Mus musculus angio-associated migratory protein DNA.
    Gene Synonym:Aamp-rs, AU040907, MGC30660, Aamp
    Species:Mouse
    Vector:pMD18-T Simple Vector
    Restriction Site:
    Tag Sequence:
    Sequence Description:Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1-2 AT/TA not causing the amino acid variation.
    Sequencing primers:( We provide with AAMP qPCR primers for gene expression analysis, MP200249 )
    Antibiotic in E.coli:Ampicillin
    Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
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