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Mouse March5 Gene ORF cDNA clone in cloning vector

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Mouse 41703 cDNA Clone Product Information
NCBI RefSeq:NM_027314.3
RefSeq ORF Size:837bp
cDNA Description:Full length Clone DNA of Mus musculus membrane-associated ring finger (C3HC4) 5.
Gene Synonym:MITOL, Marchv, Rnf153, MARCH-V, 1810015H18Rik, 2310008I22Rik, 2700055A20Rik, 5730499H23Rik, E130202O05Rik
Species:Mouse
Vector:pGEM-T Vector
Plasmid:pGEM-mMARCH5
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
41703neucleotide sequence and amino acid sequence
 
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Mouse March5 Gene ORF cDNA clone in cloning vector on other vectors
Mouse March5 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagMG52606-ACG$225
Mouse March5 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagMG52606-ACR$225
Mouse March5 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagMG52606-ANG$225
Mouse March5 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagMG52606-ANR$225
Mouse March5 Gene ORF cDNA clone expression plasmid, C-Flag tagMG52606-CF$195
Mouse March5 Gene ORF cDNA clone expression plasmid, C-His tagMG52606-CH$195
Mouse March5 Gene ORF cDNA clone expression plasmid, C-Myc tagMG52606-CM$195
Mouse March5 Gene ORF cDNA clone expression plasmid, C-HA tagMG52606-CY$195
Mouse March5 Gene ORF cDNA clone in cloning vectorMG52606-G$75
Mouse March5 Gene ORF cDNA clone expression plasmid, N-Flag tagMG52606-NF$195
Mouse March5 Gene ORF cDNA clone expression plasmid, N-His tagMG52606-NH$195
Mouse March5 Gene ORF cDNA clone expression plasmid, N-Myc tagMG52606-NM$195
Mouse March5 Gene ORF cDNA clone expression plasmid, N-HA tagMG52606-NY$195
Mouse March5 Gene ORF cDNA clone expression plasmidMG52606-UT$195
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