4-1BBL / CD137L / TNFSF9 Antibody ( FITC ) (Cytokine)

4-1BBL / CD137L / TNFSF9 Antibody Datasheet

4-1BBL / CD137L / TNFSF9 Antibody Product Information

• Immunogen: Recombinant Mouse 4-1BBL / CD137L / TNFSF9 protein (Catalog#50067-M07H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 014
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse 4-1BBL / CD137L / TNFSF9 (rM 4-1BBL / CD137L / TNFSF9; Catalog#50067-M07H; NP_033430.1; Arg 104-Glu 309) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

4-1BBL / CD137L / TNFSF9 Antibody Usage Guide

• Specificity: Mouse 4-1BBL / CD137L / TNFSF9
• Flow Cytometry:
  

  Profile of anti-TNFSF9 reactivity on Raw264.7 cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with Mouse BD Fc Block™ purified anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141) prior to staining, washed, then stained with FITC Rabbit anti-TNFSF9.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

4-1BBL / CD137L / TNFSF9 Antibody Background

4-1BB ligand (4-1BBL), also known as TNFSF9, is a member of the tumor necrosis factor (TNF) superfamily (TNFSF), and interacts with 4-1BB (CDw137) expressed on activated T cells and delivers a costimulatory signal for T cell activation and growth. Mouse 4-1BBL is a 309 amino acid (aa) residues protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that other TNFSF members, but shares only low aa sequence homology (14 - 16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart which is detected in both T and B cells of human peripheral blood. 4-1BB ligand interacts with 4-1BB and co-stimulates the latter to secrete cytokines and to proliferate, and also this interaction modulates the effector CD4+ T cell-driven immune response and cytokine production in colitis without affecting regulatory T cell function.

References

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4. Futagawa, T. et al., 2002, Int Immunol. 14 (3): 275-286.
5. Yoshida,H. et al., 2003, Cancer Immunol Immunother.52 (2): 97-106.
6. Maerten, P. et al., 2006, Clin Exp Immunol.143 (2): 228-236.