Anti-2B4 / CD244 Antibody

Cat: 10042-R025

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Anti-2B4 / CD244 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-2B4 / CD244 Antibody
Applications
WB,ELISA(Cap),IP
Species reactivity
Reacts with: Human
Specificity
Human 2B4 / CD244
Immunogen
Recombinant Human 2B4 / CD244 protein (Catalog#10042-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human 2B4 / CD244 (rh 2B4 / CD244; Catalog#10042-H08H; NP_057466.1; Met1-Arg221).
Source
Monoclonal Rabbit IgG Clone #025
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-2B4 / CD244 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
IP 1-4 μL/mg of lysate  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human 2B4 / CD244 in ELISA pair set (Catalog: # SEK10042). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10042-R031).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-2B4 / CD244 Antibody (Rabbit Monoclonal antibody) Images

Human 2B4 / CD244 Western blot (WB) 13083

Anti-2B4 rabbit monoclonal antibody at 1:500 dilution

Lane A: Daudi Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:28 kDa

(We are unsure as to the identity of these extra bands.)

Mouse LRPAP1/A2MRAP Immunoprecipitation(IP) 8229

2B4 was immunoprecipitated using:

Lane A:0.5 mg Daudi Whole Cell Lysate

2 µL anti-2B4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-2B4 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 28 kDa

Anti-2B4 / CD244 Antibody Alternative Names

Anti-2B4 Antibody;Anti-NAIL Antibody;Anti-NKR2B4 Antibody;Anti-Nmrk Antibody;Anti-SLAMF4 Antibody

2B4 / CD244 Background Information

The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Full Name
CD244 molecule, natural killer cell receptor 2B4
References
  • Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.
  • Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.
  • McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.
  • Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-2B4 / CD244 Antibody Rabbit Monoclonal ELISA(Det) 10042-R031
    Anti-2B4 / CD244 Antibody Rabbit Polyclonal ELISA 10042-RP01
    Anti-2B4 / CD244 Antibody Mouse Monoclonal ELISA 10042-MM02
    Anti-2B4 / CD244 Antibody Rabbit Polyclonal ELISA 10042-RP02
    Anti-2B4 / CD244 Antibody Rabbit Monoclonal ELISA 10042-R020
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