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14-3-3 sigma / Stratifin / YWHAS / SFN Antibody, Rabbit PAb, Antigen Affinity Purified

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Mouse YWHAS/SFN Antibody Product Information
Immunogen:Recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN protein (Catalog#50576-MNCE)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN (rM 14-3-3 sigma / Stratifin / YWHAS / SFN; Catalog#50576-MNCE; NP_061224.2; Met 1-Ser 248). 14-3-3 sigma / Stratifin / YWHAS / SFN specific IgG was purified by Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN affinity chromatography.
Mouse YWHAS/SFN Antibody Usage Guide
Specificity:Mouse 14-3-3 sigma / Stratifin / YWHAS / SFN
Application:WB, ELISA, IHC-P, IP

WB: 1-10 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mSFN. The detection limit for mSFN is approximately 0.039 ng/well.

IHC-P: 0.1-2 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Mouse YWHAS/SFN Antibody WB Application Image
Mouse YWHAS/SFN Antibody IHC Application Image
14-3-3 sigma / Stratifin / YWHAS / SFN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of mouse SFN in mouse skin with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Mouse YWHAS/SFN Antibody IP Application Image
Other YWHAS/SFN Antibody Products
Reactivity: Human  
Application: FCM  
  • Slide 1
10838-MM02-P-100
10838-MM02-P-50
100 Tests 
50 Tests 
Add to Cart
Reactivity: Human  
Application: ELISA  
    10838-RP01-100
    10838-RP01-200
    10838-RP01-400
    100 µg 
    200 µg 
     
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10838-RP02-50
      10838-RP02-100
      10838-RP02-200
      50 µg 
      100 µg 
      200 µg 
      Add to Cart
      Reactivity: Mouse  
      Application: ELISA  
        50576-RP01-100
        50576-RP01-200
        50576-RP01-400
        100 µg 
        200 µg 
         
        Add to Cart
        14-3-3 sigma/Stratifin/YWHAS Background

        14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

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        Catalog: 50576-RP02-50
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        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"