>Rabbit PAb Antibody
>14-3-3 sigma / Stratifin / YWHAS / SFN Antibody (Antigen Affinity Purified)
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14-3-3 sigma / Stratifin / YWHAS / SFN Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for 14-3-3 sigma / Stratifin / YWHAS / SFN product||Quality antibodies||Antibody production services|
|Detection limit is 2 ng/lane in WB|
|Detection limit is 0.00975 ng/well in ELISA|
14-3-3 sigma / Stratifin / YWHAS / SFN Antibody Product Information
Recombinant Human 14-3-3 sigma / Stratifin / YWHAS / SFN protein (Catalog#10838-HNCE)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human 14-3-3 sigma / Stratifin / YWHAS / SFN (rh 14-3-3 sigma / Stratifin / YWHAS / SFN; Catalog#10838-HNCE; NP_006133.1; Met 1-Ser 248). 14-3-3 sigma / Stratifin / YWHAS / SFN specific IgG was purified by Human 14-3-3 sigma / Stratifin / YWHAS / SFN affinity chromatography.
14-3-3 sigma / Stratifin / YWHAS / SFN Antibody Usage Guide
Human 14-3-3 sigma / Stratifin / YWHAS / SFN
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SFN in WB. Using a DAB detection system, the detection limit for Human SFN is approximately 2 ng/lane under reducing conditions.Use of this antibody under non-reducing conditions is not recommended.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SFN. The detection limit for Human SFN is approximately 0.00975 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
14-3-3 sigma / Stratifin / YWHAS / SFN Antibody Related Products & Topics
14-3-3 sigma / Stratifin / YWHAS / SFN Antibody Background
14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
- Mhawech, P. et al., 2005, Cell. Res. 15: 228-236.
- Leffers, H. et al., 1993, J. Mol. Biol. 231:982-998.
- Wiker, EW. et al., 2005, J. Biol. Chem. 280: 18891-18898.
- Brummer, T. et al., 2008, EMBO. J. 27: 2305-2316.
14-3-3 sigma (Stratifin, YWHAS) related areas, pathways, and other information