All 14-3-3 sigma reagents are produced in house and quality controlled, including 7 14-3-3 sigma Antibody, 42 14-3-3 sigma Gene, 2 14-3-3 sigma IPKit, 3 14-3-3 sigma Protein, 3 14-3-3 sigma qPCR. All 14-3-3 sigma reagents are ready to use.
Recombinant 14-3-3 sigma proteins are expressed by E. coli with fusion tags as N-cleavage, N-GST.
14-3-3 sigmaantibodies are validated with different applications, which are WB, ELISA, ICC/IF, IF, IP, FCM, IHC-P.
14-3-3 sigmacDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each 14-3-3 sigma of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.