Anti-14-3-3 sigma Antibody (PE)

Cat: 10838-MM02-P

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Anti-14-3-3 sigma Antibody (PE) (Mouse Monoclonal antibody) General Information

Product name
Anti-14-3-3 sigma Antibody (PE)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 sigma
Immunogen
Recombinant Human 14-3-3 sigma / Stratifin / YWHAS Protein (Catalog#10838-HNCE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 sigma / Stratifin / YWHAS (rh 14-3-3 sigma / Stratifin / YWHAS; Catalog#10838-HNCE; NP_006133.1; Met1-Ser248) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Mouse IgG1 Clone #02
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
PE
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-14-3-3 sigma Antibody (PE) (Mouse Monoclonal antibody) Validated Applications

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 sigma Antibody (PE) (Mouse Monoclonal antibody) Images

Human 14-3-3 sigma/Stratifin/YWHAS Flow Cytometry (FC) 5036
Flow cytometric analysis of Human SFN expression on HeLa cells. Cells were stained with PE-conjugated anti-Human SFN. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-14-3-3 sigma Antibody (PE) Alternative Names

Anti-YWHAS Antibody

14-3-3 sigma Background Information

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Full Name
stratifin
References
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 50576-RP01
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50576-RP02
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF,IP 10838-T54
    Anti-14-3-3 sigma Antibody Mouse Monoclonal FCM 10838-MM02
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 10838-RP01
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 10838-RP02
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