Mouse YWHAG Gene cDNA Clone / ORF Clone

YWHAG cDNA Clone Datasheet

YWHAG cDNA Clone Product Information

• Gene Bank Ref.ID: NM_018871.3
• cDNA Size: 744
• Gene Synonym: D7Bwg1348e; 14-3-3gamma; Ywhag
• Source: Mouse

YWHAG cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

YWHAG cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

YWHAG cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

YWHAG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTGGACC GCGAGCAACT GGTGCAGAAA GCCCGGCTGG CCGAGCAGGC GGAGCGCTAC
61       GACGATATGG CCGCGGCCAT GAAGAACGTG ACCGAGCTGA ACGAACCACT GTCCAATGAG
121      GAACGGAACC TCCTGTCGGT GGCCTACAAG AACGTGGTGG GGGCTCGCCG CTCCTCCTGG
181      AGGGTCATCA GCAGCATCGA GCAGAAGACG TCTGCGGACG GCAACGAGAA GAAGATCGAG
241      ATGGTCCGAG CCTACCGGGA GAAGATCGAG AAGGAGCTGG AGGCCGTGTG CCAGGACGTG
301      CTGAGCCTGC TGGACAACTA CCTGATCAAG AACTGCAGCG AGACCCAGTA CGAGAGCAAG
361      GTGTTCTACC TGAAGATGAA AGGGGACTAT TACCGTTACC TGGCAGAAGT GGCCACCGGG
421      GAGAAGAGGG CGACCGTGGT GGAGTCGTCT GAGAAGGCCT ACAGCGAAGC CCACGAGATC
481      AGCAAGGAGC ACATGCAGCC CACCCACCCC ATCCGGCTGG GCCTGGCGCT CAACTACTCG
541      GTTTTCTACT ATGAGATCCA GAACGCCCCG GAGCAAGCGT GCCACCTGGC CAAGACCGCC
601      TTCGACGACG CCATCGCCGA GCTCGACACT CTGAACGAGG ACTCCTACAA GGACTCCACT
661      CTGATCATGC AGCTGCTCCG AGACAACCTC ACGCTCTGGA CGAGCGACCA GCAAGACGAC
721      GACGGCGGTG AAGGCAACAA CTAA

 

Quality Control:cDNA sequencing results is available upon request.