Application | Dilution |
---|---|
WB | 1:500-1:2000 |
ELISA | 1:1000-1:2000 |
IP | 1-4 μL/mg of lysate |
Anti-YWHAH mouse monoclonal antibody at 1:500 dilution
Lane A: U937 Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lane C: Jurkat Whole Cell Lysate
Lane D: K562 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:28 kDaObserved band size:30 kDa
YWHAH was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
Lane B:0.5 mg k562 Whole Cell Lysate
2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-YWHAH mouse monoclonal antibody,at 1:500 dilution
Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 28 kDaObserved band size: 28 kDa