Anti-14-3-3 eta / YWHAH Antibody
Cat: 10847-MM06
Anti-14-3-3 eta / YWHAH Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-14-3-3 eta / YWHAH Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 eta / YWHAH
No cross-reactivity in ELISA with
E.coli cell lysate
No cross-reactivity in ELISA with
E.coli cell lysate
Immunogen
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #06
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 eta / YWHAH Antibody (Mouse Monoclonal antibody) Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| ELISA | 1:1000-1:2000 |
| IP | 1-4 μL/mg of lysate |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-14-3-3 eta / YWHAH Antibody (Mouse Monoclonal antibody) Images
Anti-YWHAH mouse monoclonal antibody at 1:500 dilution
Lane A: U937 Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lane C: Jurkat Whole Cell Lysate
Lane D: K562 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:28 kDaObserved band size:30 kDa
YWHAH was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
Lane B:0.5 mg k562 Whole Cell Lysate
2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-YWHAH mouse monoclonal antibody,at 1:500 dilution
Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 28 kDaObserved band size: 28 kDa
Anti-14-3-3 eta / YWHAH Antibody: Alternative Names
Anti-YWHA1 Antibody
14-3-3 eta / YWHAH Background Information
YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region which has been implicated by linkage studies in both BP and schizophrenia.
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Research Areas
- Cancer>>Signal Transduction>>Adaptor Proteins
- Cancer>>Signal Transduction>>Akt Pathway>>Adaptor Proteins in the Akt Pathway
- Cancer>>Signal Transduction>>Signaling Pathway>>Representative pathway>>Apoptosis Signaling pathway>>Apoptosis Adaptor Proteins
- Cancer>>Signal Transduction>>Signaling Pathway>>Representative pathway>>Apoptosis Signaling pathway>>Bcl-2 Proteins and Regulators
- Neuroscience>>Neurotransmission>>Intracellular Signaling>>Adapters>>Cytoplasmic
- Cancer>>Signal Transduction>>phospho-Serine/phospho-Threonine Binding Proteins
References
- Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
- Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
- Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
- Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
Related Pathways
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Death Receptor Signaling -
TNF Signaling -
Actin Dynamics Signaling Pathway -
AKT Signaling Pathway -
MAPK-Erk Pathway
