|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-YWHAE rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell Lysate
Lane B: Jurkat Whole Cell Lysate
Lane C: SHSY5Y Whole Cell Lysate
Lane D: SW480 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:29 kDa
Observed band size:32 kDa
(We are unsure as to the identity of these extra bands.)
YWHAE was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate
Lane C:0.5 mg SH-SY5Y Whole Cell Lysate4 µL anti-YWHAE rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 29 kDa
Observed band size: 34 kDa