Anti-14-3-3 epsilon Antibody

Cat: 10842-T54
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Anti-14-3-3 epsilon Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-14-3-3 epsilon Antibody
Validated applications
WB,ELISA,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 epsilon
Immunogen
Recombinant Human 14-3-3 epsilon/YWHAE Protein (Catalog#10842-HNCE)
Preparation
Produced in rabbits immunized with purified, recombinant Human 14-3-3 epsilon/YWHAE (rh 14-3-3 epsilon/YWHAE; Catalog#10842-HNCE; NP_006752.1; Met1-Gln255). 14-3-3 epsilon/YWHAE specific IgG was purified by Human 14-3-3 epsilon/YWHAE affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 epsilon Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
ICC-IF 1:300-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 epsilon Antibody (Rabbit Polyclonal antibody) Images
Human 14-3-3 epsilon/YWHAE Western blot (WB) 15720

Anti-YWHAE rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: SHSY5Y Whole Cell Lysate

Lane D: SW480 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

Human 14-3-3 epsilon/YWHAE Immunofluorescence(IF) 15721
Immunofluorescence staining of YWHAE in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human YWHAE polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human 14-3-3 epsilon/YWHAE Immunoprecipitation(IP) 15722

YWHAE was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

Lane C:0.5 mg SH-SY5Y Whole Cell Lysate

4 µL anti-YWHAE rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 34 kDa

Anti-14-3-3 epsilon Antibody Alternative Names
Anti-14-3-3E Antibody;Anti-HEL2 Antibody;Anti-KCIP-1 Antibody;Anti-MDCR Antibody;Anti-MDS Antibody
14-3-3 epsilon Background Information

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
References
  • Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.
  • Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.
  • Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 epsilon Antibody Mouse Monoclonal ELISA 10842-MM07
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