Cynomolgus 14-3-3 beta / YWHAB Protein

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein Protein Datasheet

YWHAB Protein Product Information

• Synonym: YWHAB
• Protein Construction: A DNA sequence encoding the mature form of Cynomolgus (Macaca fascicularis) YWHAB (Q4R572-2 ) (Met 2-Asn 244 ) was expressed and purified, with an initial Met.
• Source: Cynomolgus
• Expression Host: E.coli

YWHAB Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant Cynomolgus YWHAB consists of 246 amino acids and has a calculated molecular mass of 27.8 KDa. It migrates as an approximately 30 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, 10%glycerol, 0.5mM DTT , pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: YWHAB protein

YWHAB Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

YWHAB Protein Description

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), also known as 14-3-3 protein beta / alpha and protein kinase C inhibitor protein 1(KCIP1), is a member of the 14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAB has been repoted to interact with SSH1,TORC2/CRTC2, ABL1, ROR2, GAB2, and thus is invloved in the related processes, such as apoptosis. Inaddition, YWHAB may serve as a negative regulator of osteogenesis.

References

1. Mhawech P. et al., 2005, Cell Res. 15: 228-36.
2. Screaton R A. et al., 2004, Cell.119: 61-74.
3. Brummer T. et al., 2008, EMBO J. 27: 2305-16.
4. Yang X. et al., 2006, Proc Natl Acad Sci.103: 17237-42.
5. Liu Y. et al., 2007, Mol Endocrinol. 21: 3050-61.