Application | Dilution |
---|---|
WB | 1:500-1:2000 |
ELISA | 1:5000-1:10000 |
IP | 1-4 μL/mg of lysate |
YWHAB was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate
2 µL anti-YWHAB rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-YWHAB rabbit polyclonal antibody,at 1:200 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 28 kDaObserved band size: 28 kDa
Anti-YWHAB rabbit polyclonal antibody at 1:500 dilution
Lane A: Hela Whole Cell Lysate
Lane B: HepG2 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:28 kDaObserved band size:34 kDa
(We are unsure as to the identity of these extra bands.)