YWHAB Protein

14-3-3 protein beta/alpha ( YWHAB / KCIP-1 ) Protein Datasheet

YWHAB / KCIP-1 Protein Product Information

• Synonym: GW128 , HS1 , KCIP-1 , YWHAB
• Protein Construction: A DNA sequence encoding the human YWHAB ( NP_003395.1 ) ( Met 1- Asn 246 ) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus
• Source: Human
• Expression Host: E.coli

YWHAB / KCIP-1 Protein QC Testing

• Purity: > 93 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human YWHAB consists of 248 amino acids and has a predicted molecular mass of 28 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5
• SDS-PAGE:

YWHAB / KCIP-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

YWHAB / KCIP-1 Protein Description

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), also known as 14-3-3 protein beta / alpha and protein kinase C inhibitor protein 1(KCIP1), is a member of the 14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAB has been repoted to interact with SSH1,TORC2/CRTC2, ABL1, ROR2, GAB2, and thus is invloved in the related processes, such as apoptosis. Inaddition, YWHAB may serve as a negative regulator of osteogenesis.

References

1. Mhawech, P. et al., 2005, Cell. Res. 15: 228-236.
2. Screaton, R.A. et al., 2004, Cell.119:61-74
3. Brummer, T. et al., 2008, EMBO. J. 27: 2305-2316.
4. Yang, X. et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103: 17237-17242.
5. Liu, Y. et al., 2007, Mol. Endocrinol. 21: 3050-3061.